Key features and details
- APC/Cy5.5® Rat monoclonal [53-6.7] to CD8 alpha
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: APC/Cy5.5®. Ex: 650nm, Em: 690nm
- Isotype: IgG2a
Product nameAPC/Cy5.5® Anti-CD8 alpha antibody [53-6.7]
See all CD8 alpha primary antibodies
DescriptionAPC/Cy5.5® Rat monoclonal [53-6.7] to CD8 alpha
ConjugationAPC/Cy5.5®. Ex: 650nm, Em: 690nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
The details of the immunogen for this antibody are not available.
- Mouse mesenteric lymph node cells.
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
In vivo and in vitro treatment with the clone 53-6.7 monoclonal antibody effectively depletes CD8a+ cells. Clone 53-6.7 also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.1
Preservative: 0.09% Sodium azide
Constituents: 16% Sucrose, PBS
Also contains a stabilizing agent.
Concentration information loading...
Primary antibody notesIn vivo and in vitro treatment with the clone 53-6.7 monoclonal antibody effectively depletes CD8a+ cells. Clone 53-6.7 also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
Light chain typekappa
- Biotin Anti-CD8 alpha antibody [53-6.7] (ab25176)
- PE/Texas Red® Anti-CD8 alpha antibody [53-6.7] (ab25294)
- Anti-CD8 alpha antibody [53-6.7] (ab25478)
- PE Anti-CD8 alpha antibody [53-6.7] (ab25498)
- APC Anti-CD8 alpha antibody [53-6.7] (ab25499)
- FITC Anti-CD8 alpha antibody [53-6.7] (ab25676)
- PE Anti-CD8 alpha antibody [53-6.7] (ab272343)
- Cy5 ® Anti-CD8 alpha antibody [53-6.7] (ab39850)
- PE/Cy7® Anti-CD8 alpha antibody [53-6.7] (ab39853)
- PE/Cy5® Anti-CD8 alpha antibody [53-6.7] (ab95588)
Our Abpromise guarantee covers the use of ab82005 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.2µg for 106 cells.|
FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
ab82005 has been referenced in 3 publications.
- Takahashi K et al. CD4 and CD8 regulate interleukin 2 responses of T cells. Proc Natl Acad Sci U S A 89:5557-61 (1992). Depletion ; Mouse . PubMed: 1608966
- Ledbetter JA et al. T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med 152:280-95 (1980). Flow Cyt ; Mouse . PubMed: 6156984
- Ledbetter JA & Herzenberg LA Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev 47:63-90 (1979). Flow Cyt, IP ; Mouse . PubMed: 398327