APC/Cy7® Anti-CD28 antibody [CD28.2] (ab270676)

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Flow Cytometry - APC/Cy7® Anti-CD28 antibody [CD28.2] (ab270676)
  • Flow Cytometry - APC/Cy7® Anti-CD28 antibody [CD28.2] (ab270676)

Key features and details

  • APC/Cy7® Mouse monoclonal [CD28.2] to CD28
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: APC/Cy7®. Ex: 650nm, Em: 774nm
  • Isotype: IgG1

Overview

  • Product name

    APC/Cy7® Anti-CD28 antibody [CD28.2]
    See all CD28 primary antibodies

  • Description

    APC/Cy7® Mouse monoclonal [CD28.2] to CD28

  • Host species

    Mouse

  • Conjugation

    APC/Cy7®. Ex: 650nm, Em: 774nm

  • Specificity

    This antibody recognizes an extracellular epitope of CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical co-stimulatory receptor of T cells.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus corresponding to CD28. DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA.

  • Positive control

    • Flow Cyt: Human peripheral whole blood.

  • General notes

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
Application Abreviews Notes
Flow Cyt
Use 4µl for 106 cells.

The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in suspension.
Notes
Flow Cyt
Use 4µl for 106 cells.

The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in suspension.

Target

  • Function

    Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.

  • Tissue specificity

    Expressed in T-cells and plasma cells, but not in less mature B-cells.

  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD 28 antibody
      • CD28 antibody
      • CD28 antigen antibody
      • CD28 molecule antibody
      • CD28_HUMAN antibody
      • MGC138290 antibody
      • T cell antigen CD28 antibody
      • T cell specific surface glycoprotein antibody
      • T cell specific surface glycoprotein CD28 antibody
      • T-cell-specific surface glycoprotein CD28 antibody
      • TP44 antibody
      see all

    Images

    • Flow Cytometry - APC/Cy7® Anti-CD28 antibody [CD28.2] (ab270676)
      Flow Cytometry - APC/Cy7® Anti-CD28 antibody [CD28.2] (ab270676)

      Flow cytometry analysis of human peripheral whole blood labeling CD28 positive lymphocytes using ab270676 (Red). Surface staining.

      Control: Human neutrophil granulocytes (Black).

    • Flow Cytometry - APC/Cy7® Anti-CD28 antibody [CD28.2] (ab270676)
      Flow Cytometry - APC/Cy7® Anti-CD28 antibody [CD28.2] (ab270676)

      Flow cytometry analysis of human peripheral whole blood labeling CD28 using ab270676. Surface staining.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab270676? Please let us know so that we can cite the reference in this datasheet.

    ab270676 has not yet been referenced specifically in any publications.

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