APC/Cy7® Anti-Integrin alpha 2 antibody [DX5] (ab46795)

Reviews (1)Q&A (1)
Flow Cytometry - APC/Cy7® Anti-Integrin alpha 2 antibody [DX5] (ab46795)

    Key features and details

    • APC/Cy7® Rat monoclonal [DX5] to Integrin alpha 2
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: APC/Cy7®. Ex: 650nm, Em: 774nm
    • Isotype: IgM

    Overview

    • Product name

      APC/Cy7® Anti-Integrin alpha 2 antibody [DX5]
      See all Integrin alpha 2 primary antibodies

    • Description

      APC/Cy7® Rat monoclonal [DX5] to Integrin alpha 2

    • Host species

      Rat

    • Conjugation

      APC/Cy7®. Ex: 650nm, Em: 774nm

    • Specificity

      ab46795 reacts with mouse CD49b, Mr 160-kDa.

    • Tested applications

      Suitable for: Flow Cytmore details
      Unsuitable for: IHC-Fr or IHC-P

    • Species reactivity

      Reacts with: Mouse

    • Immunogen

      RIL 2 propagated NK1.1+ cells from C57BL/6 mice. (Fusion Partner Mouse SP2/0 myeloma cell line).

    • General notes

      This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab46795 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 0.2µl for 106 cells.
  • Application notes
    Is unsuitable for IHC-Fr or IHC-P.

    • Target

    • Function

      Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.

    • Sequence similarities

      Belongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.
      Contains 1 VWFA domain.

    • Domain

      The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • BR antibody
        • CD 49b antibody
        • CD49 antigen like family member B antibody
        • CD49 antigen-like family member B antibody
        • CD49b antibody
        • CD49b antigen antibody
        • Collagen receptor antibody
        • DX5 antibody
        • Glycoprotein Ia deficiency included antibody
        • GP Ia antibody
        • GP Ia deficiency, included antibody
        • GPIa antibody
        • HPA 5 included antibody
        • HPA5 included antibody
        • Human platelet alloantigen system 5 antibody
        • Integrin alpha 2 antibody
        • Integrin alpha II antibody
        • Integrin alpha-2 antibody
        • Integrin, alpha 2 (CD49B alpha 2 subunit of VLA 2 receptor) antibody
        • ITA2_HUMAN antibody
        • ITGA2 antibody
        • Platelet alloantigen Br(a), included antibody
        • Platelet antigen Br antibody
        • Platelet glycoprotein GPIa antibody
        • Platelet glycoprotein Ia antibody
        • Platelet glycoprotein Ia/IIa antibody
        • Platelet membrane glycoprotein Ia antibody
        • Platelet receptor for collagen, deficiency of, included antibody
        • Very late activation protein 2 receptor alpha 2 subunit antibody
        • VLA 2 alpha chain antibody
        • VLA 2 antibody
        • VLA 2 subunit alpha antibody
        • VLA-2 subunit alpha antibody
        • VLA2 antibody
        • VLA2 receptor alpha 2 subunit antibody
        • VLAA2 antibody
        see all

      Images

      • Flow Cytometry - APC/Cy7® Anti-Integrin alpha 2 antibody [DX5] (ab46795)
        Flow Cytometry - APC/Cy7® Anti-Integrin alpha 2 antibody [DX5] (ab46795)
        FACS analysis of B6 splenocytes, doublestained with rat anti-mouse CD49b (integrin alpha 2)-APC/Cy7 (ab46795) at 0.2 µl/106 cells and CD3-FITC.

      References (0)

      Publishing research using ab46795? Please let us know so that we can cite the reference in this datasheet.

      ab46795 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Question

      I bought your DX5 cod ab46795, but It is not clear to me if this clone is for BALB/c or for B6, as in your daata sheet is for B6 and other vendors is for BALB/c.

      Read More
      Answer

      Thank you for your e-mail.
      I have contacted the Lab which produce this antibody regarding your question:
      "The immunogen used to produce this antibody was RIL-2-propagated NK1.1+ cells from C57BL/6 mice, though this antibody stains the majority of NK cells in numerous mouse strains including Balb/c. I have attached the technical bulletin for your reference. "
      From the refference:" The antibody stains the majority of NK cells and a small subpopulation of T cells in all mouse strains tested (e.g., A/J, AKR, BALB/c, C3H/HeJ, C57BL/6, C57BL/10, C57BR, C58, CBA/Ca, CBA/J, DBA/1, DBA/2, SJL, SWR). Three-color flow cytometry analysis has revealed that most cells that express NK-1.1 (NKR-P1C) also express CD49b. However, small subsets of DX5+NK-1.1- and DX5-NK-1.1+ cells can be found, especially among the CD3+ population of T lymphocytes.
      I hope this helps.Please let us know if you have any other questions.

      Read More

      Flow Cytometry abreview for Anti-Integrin alpha 2 antibody [DX5] (Allophycocyanin/Cy7 ®)

       Good
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Mouse Cell (whole blood)
      Specification
      whole blood
      Permeabilization
      No
      Gating Strategy
      monocyte, lymphocyte and granulocyte
      Read More

      Abcam user community

      Verified customer

      Submitted May 01 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com