APC/Cy7® Anti-RP105 antibody [G28-8] (ab253257)
![Flow Cytometry - APC/Cy7® Anti-RP105 antibody [G28-8] (ab253257)](https://www.abcam.com/ps/products/253/ab253257/Images/ab253257-343731-antirp105monoclonalantibody-flowcytometry-human.jpg "Flow Cytometry - APC/Cy7® Anti-RP105 antibody [G28-8] (ab253257)")
Key features and details
- APC/Cy7® Mouse monoclonal [G28-8] to RP105
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: APC/Cy7®. Ex: 650nm, Em: 774nm
- Isotype: IgG1
Overview
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Product name
APC/Cy7® Anti-RP105 antibody [G28-8]
See all RP105 primary antibodies -
Description
APC/Cy7® Mouse monoclonal [G28-8] to RP105 -
Host species
Mouse -
Conjugation
APC/Cy7®. Ex: 650nm, Em: 774nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human RP105. Human tonsillar B cells.
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Positive control
- Flow Cyt: Human peripheral blood cells.
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General notes
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
The purified antibody is conjugated with tandem dye APC-Cy™7 under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. -
Clonality
Monoclonal -
Clone number
G28-8 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab253257 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 4µl for 106 cells. Or 4 μL per 100 μL whole blood. |
Target
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Function
May cooperate with MD-1 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) in B-cells. Leads to NF-kappa-B activation. Also involved in the life/death decision of B-cells. -
Tissue specificity
Expressed mainly on mature peripherical B cells. Detected in spleen, lymph node and appendix. Not detected in pre-B and -T cells. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 19 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4064 Human
- Omim: 602226 Human
- SwissProt: Q99467 Human
- Unigene: 87205 Human
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Alternative names
- CD180 antibody
- CD180 antigen antibody
- CD180_HUMAN antibody
see all
Images
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Flow Cytometry - APC/Cy7® Anti-RP105 antibody [G28-8] (ab253257)
Flow cytometric analysis of human peripheral blood cells labeling RP105 (CD180) with ab253257.
Surface staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab253257 has not yet been referenced specifically in any publications.
