APC/Cy7® Anti-RP105 antibody [G28-8] (ab253257)
![Flow Cytometry - APC/Cy7® Anti-RP105 antibody [G28-8] (ab253257)](https://www.abcam.com/ps/products/253/ab253257/Images/ab253257-343731-antirp105monoclonalantibody-flowcytometry-human.jpg "Flow Cytometry - APC/Cy7® Anti-RP105 antibody [G28-8] (ab253257)")
Key features and details
- APC/Cy7® Mouse monoclonal [G28-8] to RP105
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: APC/Cy7®. Ex: 650nm, Em: 774nm
- Isotype: IgG1
Overview
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Product name
APC/Cy7® Anti-RP105 antibody [G28-8]
See all RP105 primary antibodies -
Description
APC/Cy7® Mouse monoclonal [G28-8] to RP105 -
Host species
Mouse -
Conjugation
APC/Cy7®. Ex: 650nm, Em: 774nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human RP105. Human tonsillar B cells.
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Positive control
- Flow Cyt: Human peripheral blood cells.
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General notes
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
The purified antibody is conjugated with tandem dye APC-Cy™7 under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. -
Clonality
Monoclonal -
Clone number
G28-8 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab253257 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 4µl for 106 cells.
Or 4 μL per 100 μL whole blood. |
| Notes |
|---|
|
Flow Cyt
Use 4µl for 106 cells. Or 4 μL per 100 μL whole blood. |
Target
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Function
May cooperate with MD-1 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) in B-cells. Leads to NF-kappa-B activation. Also involved in the life/death decision of B-cells. -
Tissue specificity
Expressed mainly on mature peripherical B cells. Detected in spleen, lymph node and appendix. Not detected in pre-B and -T cells. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 19 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4064 Human
- Omim: 602226 Human
- SwissProt: Q99467 Human
- Unigene: 87205 Human
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Alternative names
- CD180 antibody
- CD180 antigen antibody
- CD180_HUMAN antibody
see all
Images
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Flow Cytometry - APC/Cy7® Anti-RP105 antibody [G28-8] (ab253257)
Flow cytometric analysis of human peripheral blood cells labeling RP105 (CD180) with ab253257.
Surface staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab253257 has not yet been referenced specifically in any publications.
