• Product name
    APC/Cy7® Conjugation Kit
  • Product overview

    APC/Cy7® Conjugation Kit ab102859 uses a simple and quick process to conjugate an antibody to HRP. It can also be used to conjugate other proteins or peptides.

    To conjugate an antibody to APC/Cy7® using this kit:
    - add modifier to antibody and incubate for 3 hrs
    - add quencher and incubate for 30 mins
    The conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.

    Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to APC/Cy7®.


  • Notes

    Amount and volume of antibody for conjugation to APC/Cy7®.

     Kit size   Recommended maximum
    amount of antibody
    Maximum antibody 
    30 µg   3 x 10 µg 3 x 10 µL
    100 µg   100 µg  100 µL
    300 µg   3 x 100 µg  3 x 100 µL
    1 mg   1 mg 1 mL

    1 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 5mg/ml or < 0.5 mg/ml should be diluted /concentrated.


    Buffer Requirements for Conjugation

    Buffer should be pH 6.5-8.5.

    Compatible buffer constituents 
    If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.

    50mM / 0.6% Tris1 0.1%/1% BSA2 50% glycerol
    0.1% sodium azide3 PBS Potassium phosphate
    Sodium chloride HEPES Sucrose
    Sodium citrate EDTA Trehalose

    1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
    2 1% BSA gives lower quality conjugates, BSA can also interfere with the use of the conjugated antibody in tissue staining.

    Incompatible buffer constituents

    Thiomerosal Proclin Glycine
    Arginine Glutathione DTT

    If a constituent of the buffer containing your antibody or protein is not listed above, please check the FAQ or contact us.

    Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.

  • Tested applications
    Suitable for: Conjugationmore details



Our Abpromise guarantee covers the use of ab102859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Conjugation Use at an assay dependent dilution.


  • This illustration demonstrates a general procedure and will slightly vary dependent on the conjugate used.



This product has been referenced in:
  • Oliveira BM  et al. T cells in mesenteric and subcutaneous adipose tissue of Holstein-Friesian cows. Sci Rep 9:3413 (2019). Read more (PubMed: 30833655) »
  • Correa R  et al. Extracellular vesicles carrying lactate dehydrogenase induce suicide in increased population density of Plasmodium falciparum in vitro. Sci Rep 9:5042 (2019). Read more (PubMed: 30911042) »
See all 5 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A


Thank you for your patience.

I have received an update from the lab and unfortunately they are unable to conjugate this antibody with fluorophores. Therefore the conjugation kits we provide would be the most suitable alternative. If you have any further questions just let me know.

Apologies for any inconvenience.

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Thank you for your patience.

I am still waiting for the lab to confirm whether it will be possible to conjugate the clone MMA (Anti-CD15 antibody) to APC, APC/Cy7 or Alexa fluor 647.

We have a number of conjugation kits that enable you to conjugate antibodies with APC/Cy7. Here are the links to the products.

ab102856 EasyLink APC/Cy7® Conjugation Kit (1 x 1mg APC/Cy7®)

ab102857 EasyLink APC/Cy7® Conjugation Kit (1x100µg APC/Cy7®)

ab102858 EasyLink APC/Cy7® Conjugation Kit (3x100µg APC/Cy7®)

ab102859 EasyLink APC/Cy7® Conjugation Kit (3 x 10µg APC/Cy7®)

I will provide further information about purchasing already conjugated antibodies as soon as I receive information from the lab.

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Thank you for your enquiry. I am sorry to confirm that we do not provide a customer conjugation service. We do have several conjugation kits available with different flourochromes. We have many available, including some for APC-Cy7 conjugation: ab102857 EasyLink APC/Cy7® Conjugation Kit (1x100µg APC/Cy7®) Click here (or use the following: https://www.abcam.com/index.html?datasheet=102857). ab102859 EasyLink APC/Cy7® Conjugation Kit (3 x 10µg APC/Cy7®) Click here (or use the following: https://www.abcam.com/index.html?datasheet=102859). There are many other conjugation kits which can be viewed on the following search results page from our website: https://www.abcam.com/index.html?pageconfig=searchresults&startPage=1 I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

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