APC/Cy7® Rat IgG2b, monoclonal [LFT-2] - Isotype control (ab253042)
Overview
-
Product name
APC/Cy7® Rat IgG2b, monoclonal [LFT-2] - Isotype control
See all IgG2b isotype controls -
Description
APC/Cy7® Rat IgG2b, monoclonal [LFT-2] - Isotype control -
Conjugation
APC/Cy7®. Ex: 650nm, Em: 774nm -
Specificity
ab253042 has an unknown binding specificity.
-
Tested applications
Suitable for: Flow Cytmore details -
General notes
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.16% Sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from tissue culture supernatant. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. -
Clonality
Monoclonal -
Clone number
LTF-2 -
Isotype
IgG2a -
Research areas
-
Relevance
IgG2 is divided into two subclasses; IgG2a and IgG2b. It is a glycoprotein which consists oftwo identical heavy chains (50 kDa each) and two identical light chains (25 kDa each), to give a combined mass of approximately 150 kDa. The chains are held in place by covalent disulfide bonds. Each light chain contains two immunoglobulin (Ig domains, while the heavy chains contain four Ig domains each. In the middle of each heavy chain is a relative varying portion called the “hinge region” which is unique to each IgG. This region allows for molecular flexibility and sets IgG apart from its IgG counter-parts. Properties of IgG2b include neutralization of toxins and diffusion in the extracellular space. -
Cellular localization
Secreted -
Alternative names
- gamma2b
- Ig gamma-2B chain C region
- Igh-3
see all
Associated products
-
Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab253042 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab253042 has not yet been referenced specifically in any publications.
