APC/Cy7® Rat IgG2b, monoclonal [LFT-2] - Isotype control (ab253042)
Overview
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Product name
APC/Cy7® Rat IgG2b, monoclonal [LFT-2] - Isotype control
See all IgG2b isotype controls -
Conjugation
APC/Cy7®. Ex: 650nm, Em: 774nm -
Specificity
ab253042 has an unknown binding specificity.
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Tested applications
Suitable for: Flow Cytmore details -
General notes
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.16% Sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from tissue culture supernatant. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. -
Clonality
Monoclonal -
Clone number
LTF-2 -
Isotype
IgG2a -
Research areas
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Relevance
IgG2 is divided into two subclasses; IgG2a and IgG2b. It is a glycoprotein which consists oftwo identical heavy chains (50 kDa each) and two identical light chains (25 kDa each), to give a combined mass of approximately 150 kDa. The chains are held in place by covalent disulfide bonds. Each light chain contains two immunoglobulin (Ig domains, while the heavy chains contain four Ig domains each. In the middle of each heavy chain is a relative varying portion called the “hinge region” which is unique to each IgG. This region allows for molecular flexibility and sets IgG apart from its IgG counter-parts. Properties of IgG2b include neutralization of toxins and diffusion in the extracellular space. -
Cellular localization
Secreted -
Alternative names
- gamma2b
- Ig gamma-2B chain C region
- Igh-3
see all
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab253042 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used. |
Notes |
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Flow Cyt
Use at an assay dependent concentration. Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab253042 has not yet been referenced specifically in any publications.