The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 354 kDa.
Isoform 3 may have a role in regulating the growth and differentiation of arterial smooth muscle cells.
Isoform 1 is preferentially expressed in striated muscle. Non-kinase form such as isoform 3 is predominantly expressed in the aorta. Isoform 3 appears to be expressed only in highly differentiated ASMC in normal vessel walls and down-regulated in dedifferentiated ASMC in vivo. In response to vascular injuries ASMC dedifferentiate and change from a quiescent and contractile phenotype to a proliferative and synthetic phenotype. This proliferation of vascular smooth muscle cells is one of the most prominent features of atherosclerosis.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Contains 2 fibronectin type-III domains. Contains 9 Ig-like (immunoglobulin-like) domains. Contains 2 protein kinase domains.
May be autophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.