Anti-Apelin antibody (ab125213)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-Apelin antibody
See all Apelin primary antibodies -
DescriptionRabbit polyclonal to Apelin
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide from the C terminal region of Human Apelin.
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Positive control
- U87, MCF7, HeLa, MM453 whole cell lysates.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 0.1% Dibasic monohydrogen sodium phosphate, 0.45% Sodium chloride, 2.5% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab125213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 9 kDa. The detection limit for ab125213 is approximately 1ng/lane under non-reducing and reducing conditions. |
Target
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FunctionEndogenous ligand for APJ, an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APJ. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives. The oral intake in the colostrum and the milk could have a role in the modulation of the immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior.
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Tissue specificityExpressed in the brain with highest levels in the frontal cortex, thalamus, hypothalamus and midbrain. Secreted by the mammary gland into the colostrum and the milk.
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Sequence similaritiesBelongs to the apelin family.
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Post-translational
modificationsSeveral active peptides may be produced by proteolytic processing of the peptide precursor. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 8862 Human
- Entrez Gene: 30878 Mouse
- Entrez Gene: 58812 Rat
- Omim: 300297 Human
- SwissProt: Q9ULZ1 Human
- SwissProt: Q9R0R4 Mouse
- SwissProt: Q9R0R3 Rat
- Unigene: 303084 Human
see all -
Alternative names
- AGTRL1 ligand antibody
- APEL antibody
- APEL_HUMAN antibody
see all
Images
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Western blot - Anti-Apelin antibody (ab125213)All lanes : Anti-Apelin antibody (ab125213) at 0.5 µg/ml
Lane 1 : U87 Cell lysate
Lane 2 : MCF-7 Cell Lysate
Lane 3 : HELA Cell Lysate
Lane 4 : MM453 Cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 9 kDa
Protocols
References
This product has been referenced in:
- Chen L et al. Apelin Protects Primary Rat Retinal Pericytes from Chemical Hypoxia-Induced Apoptosis. J Ophthalmol 2015:186946 (2015). WB ; Rat . Read more (PubMed: 26491547) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"