Overview

  • Product name

  • Description

    Rabbit polyclonal to Apelin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide from the C terminal region of Human Apelin.

  • Positive control

    • U87, MCF7, HeLa, MM453 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab125213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 9 kDa. The detection limit for ab125213 is approximately 1ng/lane under non-reducing and reducing conditions.

Target

  • Function

    Endogenous ligand for APJ, an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APJ. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives. The oral intake in the colostrum and the milk could have a role in the modulation of the immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior.
  • Tissue specificity

    Expressed in the brain with highest levels in the frontal cortex, thalamus, hypothalamus and midbrain. Secreted by the mammary gland into the colostrum and the milk.
  • Sequence similarities

    Belongs to the apelin family.
  • Post-translational
    modifications

    Several active peptides may be produced by proteolytic processing of the peptide precursor.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • AGTRL1 ligand antibody
    • APEL antibody
    • APEL_HUMAN antibody
    • Apelin 13 antibody
    • Apelin-13 antibody
    • APJ endogenous ligand antibody
    • Apln antibody
    • XNPEP2 antibody
    see all

Images

  • All lanes : Anti-Apelin antibody (ab125213) at 0.5 µg/ml

    Lane 1 : U87 Cell lysate
    Lane 2 : MCF-7 Cell Lysate
    Lane 3 : HELA Cell Lysate
    Lane 4 : MM453 Cell lysate

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 9 kDa

References

This product has been referenced in:

  • Kagota S  et al. Perivascular Adipose Tissue-Enhanced Vasodilation in Metabolic Syndrome Rats by Apelin and N-Acetyl?l-Cysteine-Sensitive Factor(s). Int J Mol Sci 20:N/A (2018). Read more (PubMed: 30597883) »
  • Bi F  et al. Catalpol pretreatment attenuates cardiac dysfunction following myocardial infarction in rats. Anatol J Cardiol 19:296-302 (2018). Read more (PubMed: 29724983) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Lung section)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate buffer
Permeabilization
No
Specification
Lung section
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Amreen Mughal

Verified customer

Submitted Mar 15 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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