Anti-Apelin antibody (ab125213)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
Anti-Apelin antibody
See all Apelin primary antibodies -
Description
Rabbit polyclonal to Apelin -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide from the C terminal region of Human Apelin.
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Positive control
- U87, MCF7, HeLa, MM453 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab125213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 9 kDa. The detection limit for ab125213 is approximately 1ng/lane under non-reducing and reducing conditions. |
Target
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Function
Endogenous ligand for APJ, an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APJ. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives. The oral intake in the colostrum and the milk could have a role in the modulation of the immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior. -
Tissue specificity
Expressed in the brain with highest levels in the frontal cortex, thalamus, hypothalamus and midbrain. Secreted by the mammary gland into the colostrum and the milk. -
Sequence similarities
Belongs to the apelin family. -
Post-translational
modificationsSeveral active peptides may be produced by proteolytic processing of the peptide precursor. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 8862 Human
- Entrez Gene: 30878 Mouse
- Entrez Gene: 58812 Rat
- Omim: 300297 Human
- SwissProt: Q9ULZ1 Human
- SwissProt: Q9R0R4 Mouse
- SwissProt: Q9R0R3 Rat
- Unigene: 303084 Human
see all -
Alternative names
- AGTRL1 ligand antibody
- APEL antibody
- APEL_HUMAN antibody
see all
Images
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Western blot - Anti-Apelin antibody (ab125213)All lanes : Anti-Apelin antibody (ab125213) at 0.5 µg/ml
Lane 1 : U87 Cell lysate
Lane 2 : MCF-7 Cell Lysate
Lane 3 : HELA Cell Lysate
Lane 4 : MM453 Cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 9 kDa
Protocols
References
This product has been referenced in:
- Kagota S et al. Perivascular Adipose Tissue-Enhanced Vasodilation in Metabolic Syndrome Rats by Apelin and N-Acetyl?l-Cysteine-Sensitive Factor(s). Int J Mol Sci 20:N/A (2018). Read more (PubMed: 30597883) »
- Bi F et al. Catalpol pretreatment attenuates cardiac dysfunction following myocardial infarction in rats. Anatol J Cardiol 19:296-302 (2018). Read more (PubMed: 29724983) »
