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Anti-Apelin antibody (ab125213)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (4)

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Western blot - Anti-Apelin antibody (ab125213)

    Key features and details

    • Rabbit polyclonal to Apelin
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-Apelin antibody
      See all Apelin primary antibodies
    • Description

      Rabbit polyclonal to Apelin
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse
    • Immunogen

      Synthetic peptide from the C terminal region of Human Apelin.

    • Positive control

      • U87, MCF7, HeLa, MM453 whole cell lysates.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
      Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurotransmitter
      • Neuropeptides
      • More Neuropeptides
      • Signal Transduction
      • Signaling Pathway
      • G Protein Signaling
      • GPCR
      • Cardiovascular
      • Blood
      • Blood Pressure regulation
      • Cardiovascular
      • Heart
      • Contractility
      • Inotropics
      • Metabolism
      • Types of disease
      • Obesity

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • HeLa whole cell lysate (ab29545)
    • Recombinant Protein

      • Recombinant Human Apelin protein (ab152927)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab125213 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    Use a concentration of 1 µg/ml. Predicted molecular weight: 9 kDa. The detection limit for ab125213 is approximately 1ng/lane under non-reducing and reducing conditions.
    Notes
    WB
    Use a concentration of 1 µg/ml. Predicted molecular weight: 9 kDa. The detection limit for ab125213 is approximately 1ng/lane under non-reducing and reducing conditions.

    Target

    • Function

      Endogenous ligand for APJ, an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APJ. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives. The oral intake in the colostrum and the milk could have a role in the modulation of the immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior.
    • Tissue specificity

      Expressed in the brain with highest levels in the frontal cortex, thalamus, hypothalamus and midbrain. Secreted by the mammary gland into the colostrum and the milk.
    • Sequence similarities

      Belongs to the apelin family.
    • Post-translational
      modifications

      Several active peptides may be produced by proteolytic processing of the peptide precursor.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession Q9ULZ1 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 8862 Human
      • Entrez Gene: 30878 Mouse
      • Omim: 300297 Human
      • SwissProt: Q9ULZ1 Human
      • SwissProt: Q9R0R4 Mouse
      • Unigene: 303084 Human
      • Unigene: 29262 Mouse
      • Alternative names

        • AGTRL1 ligand antibody
        • APEL antibody
        • APEL_HUMAN antibody
        • Apelin 13 antibody
        • Apelin-13 antibody
        • APJ endogenous ligand antibody
        • Apln antibody
        • XNPEP2 antibody
        see all

      Images

      • Western blot - Anti-Apelin antibody (ab125213)
        Western blot - Anti-Apelin antibody (ab125213)
        All lanes : Anti-Apelin antibody (ab125213) at 0.5 µg/ml

        Lane 1 : U87 Cell lysate
        Lane 2 : MCF-7 Cell Lysate
        Lane 3 : HELA Cell Lysate
        Lane 4 : MM453 Cell lysate

        Lysates/proteins at 40 µg per lane.

        Predicted band size: 9 kDa

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (4)

      Publishing research using ab125213? Please let us know so that we can cite the reference in this datasheet.

      ab125213 has been referenced in 4 publications.

      • Toczylowski K  et al. Plasma concentration and expression of adipokines in epicardial and subcutaneous adipose tissue are associated with impaired left ventricular filling pattern. J Transl Med 17:310 (2019). PubMed: 31533725
      • Kagota S  et al. Perivascular Adipose Tissue-Enhanced Vasodilation in Metabolic Syndrome Rats by Apelin and N-Acetyl?l-Cysteine-Sensitive Factor(s). Int J Mol Sci 20:N/A (2018). PubMed: 30597883
      • Bi F  et al. Catalpol pretreatment attenuates cardiac dysfunction following myocardial infarction in rats. Anatol J Cardiol 19:296-302 (2018). PubMed: 29724983
      • Chen L  et al. Apelin Protects Primary Rat Retinal Pericytes from Chemical Hypoxia-Induced Apoptosis. J Ophthalmol 2015:186946 (2015). WB ; Rat . PubMed: 26491547

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Western blot abreview for Anti-Apelin antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (Heart)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      100 µg
      Specification
      Heart
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 28 2019

      Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Apelin antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (PFA perfusion fixed frozen sections)
      Sample
      Rat Tissue sections (Lung section)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Sodium citrate buffer
      Permeabilization
      No
      Specification
      Lung section
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Fixative
      Formaldehyde
      Read More

      Amreen Mughal

      Verified customer

      Submitted Mar 15 2017

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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