Anti-Apelin antibody (ab59469)
Key features and details
- Rabbit polyclonal to Apelin
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Apelin antibody
See all Apelin primary antibodies -
Description
Rabbit polyclonal to Apelin -
Host species
Rabbit -
Specificity
This antibody reacts specifically with 8,5 kDa of human APLN protein. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Synthetic peptide corresponding to Human Apelin (C terminal).
Database link: Q9ULZ1 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab59469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/500.
|
Notes |
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IHC-P
1/500. |
Target
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Function
Endogenous ligand for APJ, an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APJ. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives. The oral intake in the colostrum and the milk could have a role in the modulation of the immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior. -
Tissue specificity
Expressed in the brain with highest levels in the frontal cortex, thalamus, hypothalamus and midbrain. Secreted by the mammary gland into the colostrum and the milk. -
Sequence similarities
Belongs to the apelin family. -
Post-translational
modificationsSeveral active peptides may be produced by proteolytic processing of the peptide precursor. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 282143 Cow
- Entrez Gene: 8862 Human
- Entrez Gene: 30878 Mouse
- Entrez Gene: 58812 Rat
- Omim: 300297 Human
- SwissProt: Q9TUI9 Cow
- SwissProt: Q9ULZ1 Human
- SwissProt: Q9R0R4 Mouse
see all -
Alternative names
- AGTRL1 ligand antibody
- APEL antibody
- APEL_HUMAN antibody
see all
Images
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ab59469 (1:500) staining Apelin in human placenta using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cytoplasmic/secreted regions of cells in the basal uterine glands.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Datasheets and documents
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Datasheet download
References (18)
ab59469 has been referenced in 18 publications.
- Zhang H et al. Apelin Rejuvenates Aged Human Mesenchymal Stem Cells by Regulating Autophagy and Improves Cardiac Protection After Infarction. Front Cell Dev Biol 9:628463 (2021). PubMed: 33738284
- Feng J et al. The Role of Apelin/APJ in a Mouse Model of Oxygen-induced Retinopathy. Invest Ophthalmol Vis Sci 61:47 (2020). PubMed: 32729912
- Xu C et al. ELABELA antagonizes intrarenal renin-angiotensin system to lower blood pressure and protects against renal injury. Am J Physiol Renal Physiol 318:F1122-F1135 (2020). PubMed: 32174138
- Mastrella G et al. Targeting APLN/APLNR Improves Antiangiogenic Efficiency and Blunts Proinvasive Side Effects of VEGFA/VEGFR2 Blockade in Glioblastoma. Cancer Res 79:2298-2313 (2019). PubMed: 30718358
- Yang L et al. High Apelin Level Indicates a Poor Prognostic Factor in Muscle-Invasive Bladder Cancer. Dis Markers 2019:4586405 (2019). PubMed: 30984306