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Anti-Apelin antibody - C-terminal (ab230536)

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Western blot - Anti-Apelin antibody - C-terminal (ab230536)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apelin antibody - C-terminal (ab230536)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apelin antibody - C-terminal (ab230536)

Key features and details

  • Rabbit polyclonal to Apelin - C-terminal
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-Apelin antibody - C-terminal
    See all Apelin primary antibodies
  • Description

    Rabbit polyclonal to Apelin - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide corresponding to Human Apelin (C terminal) conjugated to keyhole limpet haemocyanin.
    Database link: Q9ULZ1

  • Positive control

    • WB: HEK-293T and HeLa whole cell lysate. IHC-P: Human testis and lung tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 30% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Neuropeptides
    • More Neuropeptides
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Cardiovascular
    • Blood
    • Blood Pressure regulation
    • Cardiovascular
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    • Contractility
    • Inotropics
    • Metabolism
    • Types of disease
    • Obesity

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Recombinant Human Apelin protein (ab152927)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human Apelin protein (ab152927)

Applications

Our Abpromise guarantee covers the use of ab230536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Predicted molecular weight: 9 kDa.

Target

  • Function

    Endogenous ligand for APJ, an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APJ. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives. The oral intake in the colostrum and the milk could have a role in the modulation of the immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior.
  • Tissue specificity

    Expressed in the brain with highest levels in the frontal cortex, thalamus, hypothalamus and midbrain. Secreted by the mammary gland into the colostrum and the milk.
  • Sequence similarities

    Belongs to the apelin family.
  • Post-translational
    modifications

    Several active peptides may be produced by proteolytic processing of the peptide precursor.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q9ULZ1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 282143 Cow
    • Entrez Gene: 8862 Human
    • Entrez Gene: 30878 Mouse
    • Entrez Gene: 58812 Rat
    • Omim: 300297 Human
    • SwissProt: Q9TUI9 Cow
    • SwissProt: Q9ULZ1 Human
    • SwissProt: Q9R0R4 Mouse
    • SwissProt: Q9R0R3 Rat
    • Unigene: 303084 Human
    • Unigene: 29262 Mouse
    • Unigene: 22847 Rat
    see all
  • Alternative names

    • AGTRL1 ligand antibody
    • APEL antibody
    • APEL_HUMAN antibody
    • Apelin 13 antibody
    • Apelin-13 antibody
    • APJ endogenous ligand antibody
    • Apln antibody
    • XNPEP2 antibody
    see all

Images

  • Western blot - Anti-Apelin antibody - C-terminal (ab230536)
    Western blot - Anti-Apelin antibody - C-terminal (ab230536)
    All lanes : Anti-Apelin antibody - C-terminal (ab230536) at 1/500 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Predicted band size: 9 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apelin antibody - C-terminal (ab230536)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apelin antibody - C-terminal (ab230536)

    Formalin-fixed, paraffin-embedded human testis tissue stained for Apelin using ab230536 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apelin antibody - C-terminal (ab230536)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apelin antibody - C-terminal (ab230536)

    Formalin-fixed, paraffin-embedded human lung tissue stained for Apelin using ab230536 at 1/100 dilution in immunohistochemical analysis.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab230536? Please let us know so that we can cite the reference in this datasheet.

    ab230536 has not yet been referenced specifically in any publications.

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