Anti-Apelin antibody - C-terminal (ab230536)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Apelin antibody - C-terminal
See all Apelin primary antibodies -
Description
Rabbit polyclonal to Apelin - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Synthetic peptide corresponding to Human Apelin (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: Q9ULZ1 -
Positive control
- WB: HEK-293T and HeLa whole cell lysate. IHC-P: Human testis and lung tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab230536 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | 1/500 - 1/1000. Predicted molecular weight: 9 kDa. |
Target
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Function
Endogenous ligand for APJ, an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APJ. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives. The oral intake in the colostrum and the milk could have a role in the modulation of the immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior. -
Tissue specificity
Expressed in the brain with highest levels in the frontal cortex, thalamus, hypothalamus and midbrain. Secreted by the mammary gland into the colostrum and the milk. -
Sequence similarities
Belongs to the apelin family. -
Post-translational
modificationsSeveral active peptides may be produced by proteolytic processing of the peptide precursor. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 282143 Cow
- Entrez Gene: 8862 Human
- Entrez Gene: 30878 Mouse
- Entrez Gene: 58812 Rat
- Omim: 300297 Human
- SwissProt: Q9TUI9 Cow
- SwissProt: Q9ULZ1 Human
- SwissProt: Q9R0R4 Mouse
see all -
Alternative names
- AGTRL1 ligand antibody
- APEL antibody
- APEL_HUMAN antibody
see all
Images
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Western blot - Anti-Apelin antibody - C-terminal (ab230536)All lanes : Anti-Apelin antibody - C-terminal (ab230536) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Predicted band size: 9 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apelin antibody - C-terminal (ab230536)Formalin-fixed, paraffin-embedded human testis tissue stained for Apelin using ab230536 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apelin antibody - C-terminal (ab230536)Formalin-fixed, paraffin-embedded human lung tissue stained for Apelin using ab230536 at 1/100 dilution in immunohistochemical analysis.
Datasheets and documents
References
ab230536 has not yet been referenced specifically in any publications.
