Key features and details
- Selective, reversible ASIC3 channel blocker
- CAS Number: 713544-47-9
- Purity: > 98%
- Soluble in water to 1 mg/ml
- Form / State: Solid
- Source: Anthopleura elegantissima
Product nameAPETx2, ASIC3 channel blocker
DescriptionSelective, reversible ASIC3 channel blocker
Selective, reversible ASIC3 channel blocker (IC50 = 63 nM). Inhibits heteromeric ASIC2b and ASIC3 currents (IC50 = 117 nM) and NaV1.8 channels (IC50 = 2.6 μM). Shows analgesic effects in vivo.
SequenceGTACSCGNSKGIYWFYRPSCPTDRGYTGSCRYFLGTCCTPAD (Modifications: Disulfide bonds: 4-37, 6-30, 20-38)
Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in water to 1 mg/ml
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
APETx2 blocks inward currents of ASIC3 channels expressed in Xenopus oocytes.
Membrane potential was held at 5 mV and whole cell current was continuously recorded. ASIC3 currents were elicited by rapid exposure to pH 5 in physiological solution every 20 sec. 1 µM APETx2 (ab141849) was introduced into the bath via perfusion, resulting in a reversible inhibition of the pH dependent inward transients.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab141849 has not yet been referenced specifically in any publications.