Overview

  • Product name

  • Description

    Rabbit polyclonal to APEX2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 150-179 of Human APEX2.

  • Positive control

    • MCF7 cell line lysate; Human lung carcinoma tissue; MCF7 cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab135742 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab135742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 57 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Function as a weak apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Displays also double-stranded DNA 3'-5' exonuclease, 3'-phosphodiesterase activities. Shows robust 3'-5' exonuclease activity on 3'-recessed heteroduplex DNA and is able to remove mismatched nucleotides preferentially. Shows fairly strong 3'-phosphodiesterase activity involved in the removal of 3'-damaged termini formed in DNA by oxidative agents. In the nucleus functions in the PCNA-dependent BER pathway. Required for somatic hypermutation (SHM) and DNA cleavage step of class switch recombination (CSR) of immunoglobulin genes. Required for proper cell cycle progression during proliferation of peripheral lymphocytes.
  • Tissue specificity

    Highly expressed in brain and kidney. Weakly expressed in the fetal brain.
  • Sequence similarities

    Belongs to the DNA repair enzymes AP/ExoA family.
  • Cellular localization

    Nucleus. Cytoplasm. Mitochondrion. Together with PCNA, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
  • Information by UniProt
  • Database links

  • Alternative names

    • AP endonuclease 2 antibody
    • AP endonuclease XTH2 antibody
    • APE 2 antibody
    • APE2 antibody
    • APEX 2 antibody
    • APEX L2 antibody
    • APEX nuclease (apurinic/apyrimidinic endonuclease) 2 antibody
    • APEX nuclease 2 antibody
    • APEX nuclease like 2 antibody
    • APEX nuclease-like 2 antibody
    • APEX2 antibody
    • APEX2_HUMAN antibody
    • APEXL 2 antibody
    • APEXL2 antibody
    • Apurinic apyrimidinic endonuclease 2 antibody
    • Apurinic-apyrimidinic endonuclease 2 antibody
    • Apurinic/apyrimidinic endonuclease 2 antibody
    • Apurinic/apyrimidinic endonuclease like 2 antibody
    • C430040P13Rik antibody
    • DNA (apurinic or apyrimidinic site) lyase 2 antibody
    • DNA apurinic or apyrimidinic site lyase 2 antibody
    • DNA-(apurinic or apyrimidinic site) lyase 2 antibody
    • EC 4.2.99.18 antibody
    • OTTHUMP00000023390 antibody
    • OTTHUMP00000061908 antibody
    • XTH 2 antibody
    • XTH2 antibody
    • ZGRF2 antibody
    • Zinc finger GRF type containing 2 antibody
    see all

Images

  • Anti-APEX2 antibody (ab135742) at 1/100 dilution + MCF7 cell line lysate at 35 µg

    Predicted band size: 57 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung carcinoma tissue labelling APEX2 with ab135742 at 1/50 dilution, followed by peroxidase-conjugated secondary antibody and DAB staining.
  • Flow cytometry analysis of MCF7 cells labelling APEX2 with ab135742 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab135742 has not yet been referenced specifically in any publications.

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