Anti-ATG12 antibody - N-terminal (ab228494)
Key features and details
- Rabbit polyclonal to ATG12 - N-terminal
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATG12 antibody - N-terminal
See all ATG12 primary antibodies -
Description
Rabbit polyclonal to ATG12 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human ATG12 (N terminal). An adjuvant has been used to orchestrate/boost the immune response.
Database link: O94817 -
Positive control
- Flow Cyt (Intra): SH-SY5Y cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7 -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228494 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Use 2µg for 106 cells.
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Notes |
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Flow Cyt (Intra)
Use 2µg for 106 cells. |
Target
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Function
Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the ATG12 family. -
Domain
Shares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 9140 Human
- Omim: 609608 Human
- SwissProt: O94817 Human
- Unigene: 264482 Human
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Alternative names
- Apg12 antibody
- APG12-like antibody
- APG12L antibody
see all
Images
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Flow cytometric analysis of methanol-fixed, 0.1% Tween-20-permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cell line labeling ATG12 with ab228494 at 2 µg per 106 cells (black) compared with a Rabbit IgG Control (white). Donkey anti-Rabbit PE at 1/100 dilution was used as the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab228494 has not yet been referenced specifically in any publications.