Anti-ATG3 antibody [2C10A12] (ab233562)
Key features and details
- Mouse monoclonal [2C10A12] to ATG3
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-ATG3 antibody [2C10A12]
See all ATG3 primary antibodies -
Description
Mouse monoclonal [2C10A12] to ATG3 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human ATG3 aa 1-100. Expressed in E. coli.
Database link: Q9NT62 -
Positive control
- WB: Recombinant human ATG3 protein (amino acids 1-100). ATG3 (amino acids: 1-100) - hIgGFc transfected HEK-293 cell lysate. HeLa, K562 and THP-1 cell lysate. ICC/IF: SMMC-7721 cells. Flow Cytometry: Jurkat cells. IHC-P: Human stomach cancer and rectum cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
2C10A12 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 36 kDa.
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IHC-P |
1/200 - 1/1000.
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Flow Cyt |
1/200 - 1/400.
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ICC/IF |
1/200 - 1/1000.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 36 kDa. |
IHC-P
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/400. |
ICC/IF
1/200 - 1/1000. |
Target
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Function
E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. -
Tissue specificity
Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. -
Sequence similarities
Belongs to the ATG3 family. -
Post-translational
modificationsConjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 508571 Cow
- Entrez Gene: 64422 Human
- Entrez Gene: 67841 Mouse
- Entrez Gene: 171415 Rat
- Omim: 609606 Human
- SwissProt: Q0VCL3 Cow
- SwissProt: Q9NT62 Human
- SwissProt: Q9CPX6 Mouse
see all -
Alternative names
- 2610016C12Rik antibody
- Apg 3 antibody
- APG3 antibody
see all
Images
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All lanes : Anti-ATG3 antibody [2C10A12] (ab233562) at 1/500 dilution
Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : THP-1 (Human monocytic leukemia cell line) cell lysate
Predicted band size: 36 kDa -
SMMC-7721 cells stained for ATG3 (green) using ab233562 (1/200 dilution) in ICC/IF.
Blue: DRAQ5 fluorescent DNA dye.
Red: Actin filaments have been labeled with Alexa Fluor®555 phalloidin.
Secondary antibody: Goat anti-Mouse IgG (H+L) DyLight 488.
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Paraffin-embedded human rectum cancer tissue stained for ATG3 with ab233562 at a 1/200 dilution in immunohistochemical analysis. DAB staining.
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Flow Cytometry analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells, labeling ATG3 with ab233562 at a 1/200 dilution (green) compared to a negative control (red).
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Paraffin-embedded human stomach cancer tissue stained for ATG3 with ab233562 at a 1/200 dilution in immunohistochemical analysis. DAB staining.
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All lanes : Anti-ATG3 antibody [2C10A12] (ab233562) at 1/500 dilution
Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : ATG3 (amino acids: 1-100) - hIgGFc transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Predicted band size: 36 kDa -
Anti-ATG3 antibody [2C10A12] (ab233562) at 1/500 dilution + Recombinant human ATG3 protein (amino acids 1-100)
Predicted band size: 36 kDa(Expected MW is 37.3 kDa)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab233562 has not yet been referenced specifically in any publications.