Overview

  • Product name

  • Description

    Rabbit polyclonal to APG5L/ATG5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow, Pig
  • Immunogen

    Recombinant fragment within Human APG5L/ATG5 (internal sequence). The exact sequence is proprietary.
    Database link: Q9H1Y0

  • Positive control

    • ICC/IF HeLa cells and thapsigargin- or chloroquine-treated HepG2 cells. IHC-P: Mouse brain tissue. WB: NT2/D1 and PC-3 whole cell lysate; HeLa cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab228668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 32 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. The ATG12-ATG5 conjugate also negatively regulates the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Also plays a role in translation or delivery of incoming viral RNA to the translation apparatus. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.
    May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD.
  • Tissue specificity

    Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.
  • Sequence similarities

    Belongs to the ATG5 family.
  • Post-translational
    modifications

    Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.
    Acetylated by EP300.
  • Cellular localization

    Cytoplasm. Preautophagosomal structure membrane. Colocalizes with nonmuscle actin. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.
  • Information by UniProt
  • Database links

  • Alternative names

    • APG 5 antibody
    • APG 5L antibody
    • APG5 antibody
    • APG5 autophagy 5 like antibody
    • APG5 like antibody
    • APG5-like antibody
    • APG5L antibody
    • Apoptosis specific protein antibody
    • Apoptosis-specific protein antibody
    • ASP antibody
    • ATG 5 antibody
    • Atg5 antibody
    • ATG5 autophagy related 5 homolog antibody
    • ATG5_HUMAN antibody
    • Autophagy protein 5 antibody
    • Autophagy related 5 antibody
    • hAPG5 antibody
    • Homolog of S Cerevisiae autophagy 5 antibody
    • OTTHUMP00000040507 antibody
    see all

Images

  • All lanes : Anti-APG5L/ATG5 antibody (ab228668) at 1/1000 dilution

    Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 50 µM chloroquine for 24 hours, whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 32 kDa



    15% SDS-PAGE

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for APG5L/ATG5 (green) using ab228668 (1/1000 dilution) in ICC/IF. Cells mock (left panel) and treated with 50 μM chloroquine for 24 hours (right panel) were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain: Phalloidin, an F-actin marker (red).

  • Paraffin-embedded mouse brain tissue stained for APG5L/ATG5 with ab228668 at 1/400 dilution in immunohistochemical analysis.

  • All lanes : Anti-APG5L/ATG5 antibody (ab228668) at 1/1000 dilution

    Lane 1 : NT2/D1 (human embryonal carcinoma cell line) whole cell lysate
    Lane 2 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 32 kDa



    12% SDS-PAGE

  • HepG2 (human liver hepatocellular carcinoma cell line) cells stained for APG5L/ATG5 (green) using ab228668 (1/500 dilution) in ICC/IF. Cells were treated with 3 μM thapsigargin 16 hours (right panels) and mock (left panels) and were fixed in ice-cold methanol for 10 minutes, permeabilized with cooled acetone for 2 minutes.

    Nuclear counterstain: Hoechst 33342 (blue).

References

This product has been referenced in:

  • Ma C  et al. Emodin induces apoptosis and autophagy of fibroblasts obtained from patient with ankylosing spondylitis. Drug Des Devel Ther 13:601-609 (2019). Read more (PubMed: 30809091) »
  • Tang Y  et al. Overexpression of ribonuclease inhibitor induces autophagy in human colorectal cancer cells via the Akt/mTOR/ULK1 pathway. Mol Med Rep 19:3519-3526 (2019). Read more (PubMed: 30896869) »
See all 2 Publications for this product

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