Product nameAnti-APG5L/ATG5 antibody [EPR4797]
See all APG5L/ATG5 primary antibodies
DescriptionRabbit monoclonal [EPR4797] to APG5L/ATG5
Tested applicationsSuitable for: Flow Cyt, WB, IHC-P, ICCmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human APG5L/ATG5 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q9H1Y0
- THP1, Raji, HeLa, HT1080 and Human fetal kidney lysates; Human breast carcinoma and Human kidney tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 50% Glycerol, 0.05% BSA
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109490 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/1000 - 1/10000. Predicted molecular weight: 32 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC||1/100 - 1/250.|
FunctionInvolved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. The ATG12-ATG5 conjugate also negatively regulates the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Also plays a role in translation or delivery of incoming viral RNA to the translation apparatus. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.
May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD.
Tissue specificityUbiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.
Sequence similaritiesBelongs to the ATG5 family.
modificationsConjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.
Acetylated by EP300.
Cellular localizationCytoplasm. Preautophagosomal structure membrane. Colocalizes with nonmuscle actin. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.
- Information by UniProt
- APG 5 antibody
- APG 5L antibody
- APG5 antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: APG5L/ATG5 knockout HAP1 cell lysate (20 µg)
Lane 3: Raji cell lysate (20 µg)
Lane 4: Jeg-3 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109490 observed at 52 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109490 was shown to specifically react with APG5L/ATG5 when APG5L/ATG5 knockout samples were used. Wild-type and APG5L/ATG5 knockout samples were subjected to SDS-PAGE. ab109490 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
All lanes : Anti-APG5L/ATG5 antibody [EPR4797] (ab109490) at 1/1000 dilution
Lane 1 : THP1 cell lysate
Lane 2 : Raji cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HT1080 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 32 kDa
Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling APG5L/ATG5 with purified ab109490 at 1/250 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Anti-APG5L/ATG5 antibody [EPR4797] (ab109490) at 1/1000 dilution + Human fetal kidney lysate at 10 µg
Predicted band size: 32 kDa
Upper band shows APG5L/ATG5 conjugated to ATG12.
ab109490, at 1/100 dilution, staining APG5L/ATG5 in paraffin-embedded Human breast carcinoma by Immunohistochemistry.
ab109490, at 1/100 dilution, staining APG5L/ATG5 in paraffin-embedded Human kidney by Immunohistochemistry.
This product has been referenced in:
- Peng Y et al. TRIM28 activates autophagy and promotes cell proliferation in glioblastoma. Onco Targets Ther 12:397-404 (2019). Read more (PubMed: 30655676) »
- Zhu H et al. ALKBH5 inhibited autophagy of epithelial ovarian cancer through miR-7 and BCL-2. J Exp Clin Cancer Res 38:163 (2019). Read more (PubMed: 30987661) »