Overview

  • Product name

  • Description

    Rabbit polyclonal to APG8A/ATG8A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Arabidopsis thaliana
    Predicted to work with: Human
  • Immunogen

    Recombinant full length protein corresponding to APG8A/ATG8A.

  • General notes

     This product was previously labelled as APG8A

     

Properties

Applications

Our Abpromise guarantee covers the use of ab77003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 14 kDa.
IHC-Fr Use at an assay dependent concentration. PubMed: 24463818

Target

  • Relevance

    APG8A is involved in cytoplasm to vacuole transport (Cvt) vesicles and autophagosomes formation. It may mediate the delivery of the vesicles and autophagosomes to the vacuole via the microtubule cytoskeleton.
  • Cellular localization

    Cytoplasmic vesicle › cvt vesicle membrane; Lipid-anchor. Cytoplasmic vesicle › autophagosome membrane; Lipid-anchor. Vacuole membrane; Lipid-anchor. Note= Membrane-associated through a lipid anchor.
  • Database links

    • Alternative names

      • APG8A (autophagy 8A) antibody
      • AT4G21980 antibody
      • ATG8 antibody
      • ATG8A antibody
      • Autophagy 8A antibody
      • Autophagy related protein 8a antibody
      • Autophagy related ubiquitin like modifier ATG8a antibody
      • F1N20.80 antibody
      • F1N20_80 antibody
      see all

    Images

    • All lanes : Anti-APG8A/ATG8A antibody (ab77003) at 1/500 dilution

      Lane 1 : Arabidopsis whole plant extract
      Lane 2 : Maize whole plant extract

      Secondary
      All lanes : HRP-conjugated anti-rabbit IgG. at 1/20000 dilution

      Predicted band size: 14 kDa

    References

    This product has been referenced in:

    • Huang L  et al. Autophagy regulates glucose-mediated root meristem activity by modulating ROS production in Arabidopsis. Autophagy 15:407-422 (2019). Read more (PubMed: 30208757) »
    • Zhao L  et al. Autophagy contributes to sulfonylurea herbicide tolerance via GCN2-independent regulation of amino acid homeostasis. Autophagy 14:702-714 (2018). Read more (PubMed: 29377765) »
    See all 10 Publications for this product

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A

    Application
    Western blot
    Sample
    Plants Tissue lysate - other (leaves, roots or whole seedlings)
    Gel Running Conditions
    Non-reduced Denaturing (15% PA running gel)
    Loading amount
    60000 µg
    Treatment
    starvation in presence of 20 micromol L-1 E64 for 48hrs
    Specification
    leaves, roots or whole seedlings
    Blocking step
    Blotto B reagent (Santa Cruz Biotech) in TBS as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 4°C

    Dr. Olga Voitsekhovskaja

    Verified customer

    Submitted Oct 07 2016

    Answer

    We have not tested this antibody with Barley samples however as this antibody supposed to react with plant samples hence the possibility of its cross reactivity with Barley is quite high.

    Read More

    Answer

    Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

    To our knowledge, ab77003 has not been tested inimmunogold EM. Therefore, I can offer a discount off a future purchase if you buy ab77003 now, test it in immunogold EM and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of ab77003 off a future order.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab77003 in immunogold EM. I will then send a discount code. This code must be issued before purchasing ab77003 so please wait for my reply before ordering.

    2. Purchase ab77003 either by phone, fax, or online (www.abcam.com).

    3. Test it in immunogold EM.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyproductordered and the discount code is valid for 4 months after issue.
    Please remember that submission of the Abreview is sufficient for the discount code to become active.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab77003 turns out to be unsuitable for immunogold EM, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Answer

    Thank you very much for contacting us.
    Apparently, Chung and colleagues performed their SDS-PAGE with and without 6 M urea, as reported in their publication (attached). You may find details about this protocol in the Materials and Methods section, or could enquire with the authors, if you would like to gain more information.
    To our knowledge though, customised protocols are not required for this antibody and it should work with the standard protocols, which you may find attached or on the datasheet (Protocols tab) or on our website https://www.abcam.com/index.html?pageconfig=popular_protocols.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question
    Answer

    Thank you for your enquiry. A full length, 6xHis tagged protein was used as the immunogen to create ab77003. I hope this is helpful. Please contact us again if you need any further information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up