Key features and details
- Rabbit polyclonal to APH
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-APH antibody
DescriptionRabbit polyclonal to APH
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig
Recombinant fragment corresponding to Human APH aa 201-500.
IKGDQFVFYEDWGENMVSKSIPVLCVLDVESGNISVLEGVPENVSPGQAF WAPGDAGVVFVGWWHEPFRLGIRFCTNRRSALYYVDLIGGKCELLSDDSL AVSSPRLSPDQCRIVYLQYPSLIPHHQCSQLCLYDWYTKVTSVVVDVVPR QLGENFSGIYCSLLPLGCWSADSQRVVFDSAQRSRQDLFAVDTQVGTVTS LTAGGSGGSWKLLTIDQDLMVAQFSTPSLPPTLKVGFLPSAGKEQSVLWV SLEEAEPIPDIHWGIRVLQPPPEQENVQYAGLDFEAILLQPGSPPDKTQV
Database link: P13798
- WB: HeLa whole cell lysate; Mouse liver tissue lysate. IHC-P: Human renal and colon cancer tissue. ICC/IF: HeLa cells.
This product was previously labelled as AARE
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236608 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/20 - 1/200.|
|WB||1/2000 - 1/5000.|
FunctionThis enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser.
Sequence similaritiesBelongs to the peptidase S9C family.
- Information by UniProt
- AARE antibody
- ACPH antibody
- ACPH_HUMAN antibody
All lanes : Anti-APH antibody (ab236608) at 1/2000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Mouse liver tissue lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling ab236608 using ab236608 at 1/100 dilution in ICC/IF. Secondary antibody was an Alexa-Fluor®488-conjugated goat anti-rabbit IgG (H+L).
Paraffin-embedded human renal tissue stained for APH using ab236608 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human colon cancer tissue stained for APH using ab236608 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236608 has not yet been referenced specifically in any publications.