Key features and details
- Rabbit polyclonal to APH1a - Aminoterminal end
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-APH1a antibody - Aminoterminal end
See all APH1a primary antibodies
DescriptionRabbit polyclonal to APH1a - Aminoterminal end
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic 13 amino acid peptide from near the amino terminus of human APH1a.
- RAW264.7 cell lysate
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab62521 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 29 kDa).|
FunctionEssential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex.
Tissue specificityWidely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain. Isoform 1 and isoform 2 are nearly expressed at the same level.
Sequence similaritiesBelongs to the APH-1 family.
Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus > Golgi stack membrane. Predominantly located in the endoplasmic reticulum and in the cis-Golgi.
- Information by UniProt
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Lane 1 : Anti-APH1a antibody - Aminoterminal end (ab62521) at 0.5 µg/ml
Lane 2 : Anti-APH1a antibody - Aminoterminal end (ab62521) at 1 µg/ml
Lane 3 : Anti-APH1a antibody - Aminoterminal end (ab62521) at 2 µg/ml
All lanes : RAW264.7 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 29 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
ab62521 has not yet been referenced specifically in any publications.