Key features and details
- Rabbit polyclonal to APJ Receptor
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-APJ Receptor antibody
DescriptionRabbit polyclonal to APJ Receptor
Tested applicationsSuitable for: IHC-P, WB, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human
- Rat ovary tissue; Mouse heart lysate; Mouse splenocytes.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab214369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/1000. Predicted molecular weight: 43 kDa.|
|Flow Cyt||1/20 - 1/100.|
FunctionReceptor for apelin coupled to G proteins that inhibit adenylate cyclase activity. Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia.
Tissue specificityWidely expressed in the brain, in glial cells, astrocytes and neuronal subpopulations, as well as in the spleen, thymus, ovary, small intestine and colon.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- AGTR L1 antibody
- AGTRL 1 antibody
- AGTRL1 antibody
All lanes : Anti-APJ Receptor antibody (ab214369) at 1/300 dilution
Lane 1 : Mouse cerebrum lysate
Lane 2 : Mouse heart lysate
All lanes : IRDye800CW Goat Anti-Rabbit IgG at 1/200000 dilution
Predicted band size: 43 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat ovary tissue labeling APJ Receptor with ab214369 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Anti-APJ Receptor antibody (ab214369) at 1/300 dilution + Mouse heart lysate
Goat anti-rabbit IgG (H+L), HRP conjugated at 1/500 dilution
Predicted band size: 43 kDa
Flow cytometric analysis of Mouse splenocytes labeling APJ Receptor with ab 214369 at 1/100 dilution for 30 minutes, followed by incubation with a FITC conjugated secondary antibody for 30 minutes (green) compared to control cells (blue), secondary antibody only (light blue) and isotype control (orange).
ab214369 has been referenced in 4 publications.
- Bülbül M et al. Central apelin administration and restraint stress induce hypothalamic cholecystokinin release via the APJ receptor. J Neuroendocrinol 30:e12635 (2018). PubMed: 30044523
- Bi F et al. Catalpol pretreatment attenuates cardiac dysfunction following myocardial infarction in rats. Anatol J Cardiol 19:296-302 (2018). PubMed: 29724983
- Bülbül M et al. Apelin-13 inhibits gastric motility through vagal cholinergic pathway in rats. Am J Physiol Gastrointest Liver Physiol 314:G201-G210 (2018). PubMed: 29025730
- Patel SJ et al. Identification of essential genes for cancer immunotherapy. Nature 548:537-542 (2017). WB . PubMed: 28783722