Overview

  • Product name

    Anti-APJ Receptor antibody
  • Description

    Rabbit polyclonal to APJ Receptor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide within Human APJ Receptor aa 52-66 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    TVFRSSREKRRSADI


    Database link: P35414

  • Positive control

    • Rat ovary tissue; Mouse heart lysate; Mouse splenocytes.

Properties

Applications

Our Abpromise guarantee covers the use of ab214369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/1000. Predicted molecular weight: 43 kDa.
Flow Cyt 1/20 - 1/100.

Target

  • Function

    Receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity. Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia.
  • Tissue specificity

    Widely expressed in the brain, in glial cells, astrocytes and neuronal subpopulations, as well as in the spleen, thymus, ovary, small intestine and colon.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AGTR L1 antibody
    • AGTRL 1 antibody
    • AGTRL1 antibody
    • Angiotensin II receptor like 1 antibody
    • Angiotensin receptor like 1 antibody
    • Angiotensin receptor-like 1 antibody
    • Apelin receptor antibody
    • APJ (apelin) receptor antibody
    • APJ antibody
    • APJ receptor antibody
    • APJ_HUMAN antibody
    • APJR antibody
    • APLNR antibody
    • B78 antibody
    • FLJ90771 antibody
    • G protein coupled receptor APJ antibody
    • G-protein coupled receptor APJ antibody
    • G-protein coupled receptor HG11 antibody
    • GPCR34 antibody
    • HG 11 antibody
    • HG11 antibody
    • HG11 orphan receptor antibody
    • MGC45246 antibody
    • MSR antibody
    • msr/apj antibody
    see all

Images

  • All lanes : Anti-APJ Receptor antibody (ab214369) at 1/300 dilution

    Lane 1 : Mouse cerebrum lysate
    Lane 2 : Mouse heart lysate

    Secondary
    All lanes : IRDye800CW Goat Anti-Rabbit IgG at 1/200000 dilution

    Predicted band size: 43 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat ovary tissue labeling APJ Receptor with ab214369 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

  • Anti-APJ Receptor antibody (ab214369) at 1/300 dilution + Mouse heart lysate

    Secondary
    Goat anti-rabbit IgG (H+L), HRP conjugated at 1/500 dilution

    Predicted band size: 43 kDa

  • Flow cytometric analysis of Mouse splenocytes labeling APJ Receptor with ab 214369 at 1/100 dilution for 30 minutes, followed by incubation with a FITC conjugated secondary antibody for 30 minutes (green) compared to control cells (blue), secondary antibody only (light blue) and isotype control (orange).

References

This product has been referenced in:

  • Bülbül M  et al. Central apelin administration and restraint stress induce hypothalamic cholecystokinin release via the APJ receptor. J Neuroendocrinol 30:e12635 (2018). Read more (PubMed: 30044523) »
  • Bi F  et al. Catalpol pretreatment attenuates cardiac dysfunction following myocardial infarction in rats. Anatol J Cardiol 19:296-302 (2018). Read more (PubMed: 29724983) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Flow Cytometry
Sample
Human Cell (293FT transduce with hAPJ expression plasmid)
Permeabilization
No
Gating Strategy
Singlets and hereafter the main cell population
Specification
293FT transduce with hAPJ expression plasmid
Preparation
Cell harvesting/tissue preparation method: Cells are treated with trypsin and washed in PBS before adding the antibodies.
Sample buffer: PBS
Fixation
No fixation

Abcam user community

Verified customer

Submitted May 16 2017

Answer

I can confirm that on the individual datasheets, we list the applications and species that have been tested. These are covered by the Abpromise guarantee.  If there are any species or applications not listed, it means they have not yet been tested. If they have been tested but found not to be successful (which happens very rarely), we will also add a note to state this.


Therefore, I can confirm that ab66218 has not yet been tested in mouse.


Regarding the flow cytometry protocol for ab214369Anti-APJ Receptor antibody, I have copied the general flow cytometry procedure from the testing laboratory below. Please note this will be a guideline only and may require some individual optimization.
 


Flow Cytometry Protocol


Harvest and wash the cells and determine the total cell number.


Resuspend the cells to approximately 1x106 cells/mL in ice cold PBS. Add the appropriate volume of paraformaldehyde to obtain a final concentration of 4%.


Fix for 10 minutes in a 37°C water bath.


Wash cells twice with PBS containing 0.5% BSA.

Note: For extracellular staining with antibodies that do not require permeabilization, proceed to step 8; for intracellular staining, proceed to step 5.


Permeabilization


Add ice-cold 90% methanol (approximately 1mL per 1x106 cells) and vortex.


Permeabilize for a minimum of 10 minutes on ice.


Immunostaining and Analysis


Wash cells twice with PBS containing 0.5%BSA.


Resuspend 1x106 cells in 100μL PBS containing 0.5%BSA.


Add the primary antibody and incubate for 1 hour at room temperature (RT).


Wash cells twice with PBS containing 0.5%BSA.

Note: If using a fluorescent primary conjugated antibody, skip to step 14.


Resuspend cells in fluorochrome conjugated secondary antibody diluted in PBS containing 0.5% BSA.


Incubate for 30 minutes at RT.


Wash cells twice with PBS containing 0.5% BSA.


Resuspend cells in 0.5mL PBS and analyze on flow cytometer.

Read More

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