Overview

  • Product name

  • Description

    Rabbit polyclonal to APLF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human, African green monkey
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human APLF. Derived from around the phosphorylation site of serine 116 (R-N-S(p)-Q-V).
    Database link: Q8IW19

  • Positive control

    • Human brain tissue, HT29 and COS7 cell extracts.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 0.87% Sodium chloride, 50% Glycerol

    PBS is without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab196502 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab196502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/500 - 1/3000. Predicted molecular weight: 57 kDa.

Target

  • Function

    Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage.
  • Sequence similarities

    Belongs to the APLF family.
    Contains 1 FHA-like domain.
    Contains 2 PBZ-type zinc fingers.
  • Domain

    The PBZ-type zinc fingers (also named CYR) mediate non-covalent poly(ADP-ribose)-binding. Poly(ADP-ribose)-binding is dependent on the presence of zinc and promotes its recruitment to DNA damage sites.
    The FHA-like domain mediates interaction with XRCC1 and XRCC4.
  • Post-translational
    modifications

    Poly-ADP-ribosylated. In addition to binding non covalently poly(ADP-ribose) via its PBZ-type zinc fingers, the protein is also covalently poly-ADP-ribosylated by PARP1.
    Phosphorylated in an ATM-dependent manner upon double-strand DNA break.
  • Cellular localization

    Nucleus. Cytoplasm > cytosol. Localizes to DNA damage sites. Accumulates at single-strand breaks and double-strand breaks via the PBZ-type zinc fingers.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2010301N04Rik antibody
    • AI452191 antibody
    • Aplf antibody
    • APLF_HUMAN antibody
    • Aprataxin and PNK-like factor antibody
    • Apurinic-apyrimidinic endonuclease APLF antibody
    • C2orf13 antibody
    • PNK and APTX like FHA protein antibody
    • PNK and APTX-like FHA domain-containing protein antibody
    • RGD1565557 antibody
    • XIP1 antibody
    • XRCC1 interacting protein 1 antibody
    • XRCC1-interacting protein 1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling APLF with ab196502 at 1/50 dilution. The image on the right has been pre-incubated with the blocking peptide.

  • All lanes : Anti-APLF antibody (ab196502) at 1/500 dilution

    Lane 1 : HT29 cell extract
    Lane 2 : HT29 cell extract with systhesized peptide

    Predicted band size: 57 kDa

  • All lanes : Anti-APLF antibody (ab196502) at 1/500 dilution

    Lane 1 : COS7 cell extracts with systhesized peptide
    Lane 2 : COS7 cell extracts

    Predicted band size: 57 kDa

References

ab196502 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab196502.
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