Anti-APLF antibody (ab196502)
Key features and details
- Rabbit polyclonal to APLF
- Suitable for: IHC-P, WB
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
-
Product name
Anti-APLF antibody
See all APLF primary antibodies -
Description
Rabbit polyclonal to APLF -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human, African green monkey
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human APLF. Derived from around the phosphorylation site of serine 116 (R-N-S(p)-Q-V).
Database link: Q8IW19 -
Positive control
- Human brain tissue, HT29 and COS7 cell extracts.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab196502 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196502 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/50 - 1/100.
|
|
WB |
1/500 - 1/3000. Predicted molecular weight: 57 kDa.
|
Notes |
---|
IHC-P
1/50 - 1/100. |
WB
1/500 - 1/3000. Predicted molecular weight: 57 kDa. |
Target
-
Function
Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage. -
Sequence similarities
Belongs to the APLF family.
Contains 1 FHA-like domain.
Contains 2 PBZ-type zinc fingers. -
Domain
The PBZ-type zinc fingers (also named CYR) mediate non-covalent poly(ADP-ribose)-binding. Poly(ADP-ribose)-binding is dependent on the presence of zinc and promotes its recruitment to DNA damage sites.
The FHA-like domain mediates interaction with XRCC1 and XRCC4. -
Post-translational
modificationsPoly-ADP-ribosylated. In addition to binding non covalently poly(ADP-ribose) via its PBZ-type zinc fingers, the protein is also covalently poly-ADP-ribosylated by PARP1.
Phosphorylated in an ATM-dependent manner upon double-strand DNA break. -
Cellular localization
Nucleus. Cytoplasm > cytosol. Localizes to DNA damage sites. Accumulates at single-strand breaks and double-strand breaks via the PBZ-type zinc fingers. - Information by UniProt
-
Database links
- Entrez Gene: 200558 Human
- Entrez Gene: 72103 Mouse
- Entrez Gene: 500247 Rat
- Omim: 611035 Human
- SwissProt: Q8IW19 Human
- SwissProt: Q9D842 Mouse
- Unigene: 720369 Human
- Unigene: 31770 Mouse
-
Alternative names
- 2010301N04Rik antibody
- AI452191 antibody
- Aplf antibody
see all
Images
-
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling APLF with ab196502 at 1/50 dilution. The image on the right has been pre-incubated with the blocking peptide.
-
All lanes : Anti-APLF antibody (ab196502) at 1/500 dilution
Lane 1 : HT29 cell extract
Lane 2 : HT29 cell extract with systhesized peptide
Predicted band size: 57 kDa -
All lanes : Anti-APLF antibody (ab196502) at 1/500 dilution
Lane 1 : COS7 cell extracts with systhesized peptide
Lane 2 : COS7 cell extracts
Predicted band size: 57 kDa
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab196502 has been referenced in 1 publication.
- Richter C et al. Rewiring E2F1 with classical NHEJ via APLF suppression promotes bladder cancer invasiveness. J Exp Clin Cancer Res 38:292 (2019). PubMed: 31287003