Anti-apLLP antibody (ab33569)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-apLLP antibody
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DescriptionRabbit polyclonal to apLLP
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Aplysia
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Immunogen
Recombinant full length protein (Aplysia) with 6xHis tag
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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PurityWhole antiserum
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab33569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/2500. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). | |
| ICC/IF | Use a concentration of 1 µg/ml. |
Target
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RelevanceApLLP is a LAPS18-like protein found in Aplysia, a marine snail used by neurobiologists as a model organism for studying learning and memory. ApLLP is thought to play a critical role in behavioural sensitization and long term facilitation. It has been found to function as a neural activity-induced transcriptional activator in the switch from short term facilitation to long term facilitation.
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Cellular localizationNuclear
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Alternative names
- LAPS18-like antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-apLLP antibody (ab33569)Aplysia sensory neuron stained with ab33569 anti ApLLP antibody at a dilution of 1 ug/ml (red). Secondary antibody was anti-rabbit IgG Cy-3 conjugated. ApLLP is found in nucleus (mainly in nucleoli). -
Western blot - Anti-apLLP antibody (ab33569)Lane 1 : pre immune serum at 1/1000 dilution
Lane 2 : Anti-apLLP antibody (ab33569) at 1/1000 dilution
All lanes : Recombinant apLLP with 6x His tag
Predicted band size: 20 kDa
Observed band size: 20 kDa
Datasheets and documents
References
This product has been referenced in:
- Kim H et al. A nucleolar protein ApLLP induces ApC/EBP expression required for long-term synaptic facilitation in aplysia neurons. Neuron 49:707-18 (2006). Read more (PubMed: 16504946) »
