Anti-APLP1 antibody (ab192481)
Key features and details
- Rabbit polyclonal to APLP1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-APLP1 antibody
See all APLP1 primary antibodies -
Description
Rabbit polyclonal to APLP1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant full length protein corresponding to Human APLP1 aa 1 to the C-terminus.
Database link: P51693 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
purity is > 95% (by SDS-PAGE). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192481 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 72 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 72 kDa. |
Target
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Function
May play a role in postsynaptic function. The C-terminal gamma-secretase processed fragment, ALID1, activates transcription activation through APBB1 (Fe65) binding (By similarity). Couples to JIP signal transduction through C-terminal binding. May interact with cellular G-protein signaling pathways. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I.
The gamma-CTF peptide, C30, is a potent enhancer of neuronal apoptosis. -
Tissue specificity
Expressed in the cerebral cortex where it is localized to the postsynaptic density (PSD). -
Sequence similarities
Belongs to the APP family. -
Domain
The NPXY sequence motif found in many tyrosine-phosphorylated proteins is required for the specific binding of the PID domain. However, additional amino acids either N- or C-terminal to the NPXY motif are often required for complete interaction. The NPXY site is also involved in clathrin-mediated endocytosis. -
Post-translational
modificationsProteolytically cleaved by caspases during neuronal apoptosis. Cleaved, in vitro, at Asp-620 by caspase-3.
N- and O-glycosylated. O-glycosylation with core 1 or possibly core 8 glycans. Glycosylation on Ser-227 is the preferred site to Thr-228. -
Cellular localization
Cell membrane and Cytoplasm. C-terminally processed in the Golgi complex. - Information by UniProt
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Database links
- Entrez Gene: 11803 Mouse
- SwissProt: Q03157 Mouse
- Unigene: 2381 Mouse
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Alternative names
- AMYLOID BETA A4 PRECURSOR-LIKE PROTEIN 1 antibody
- AMYLOID PRECURSOR-LIKE PROTEIN antibody
- Amyloid-like protein 1 precursor antibody
see all
Images
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Anti-APLP1 antibody (ab192481) at 1/500 dilution + Mouse Brain lysate
Predicted band size: 72 kDa
Observed band size: 97 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab192481 has been referenced in 2 publications.
- Simoes S et al. Tau and other proteins found in Alzheimer's disease spinal fluid are linked to retromer-mediated endosomal traffic in mice and humans. Sci Transl Med 12:N/A (2020). PubMed: 33239387
- Paschkowsky S et al. Alternative Processing of the Amyloid Precursor Protein Family by Rhomboid Protease RHBDL4. J Biol Chem 291:21903-21912 (2016). PubMed: 27563067