Overview

  • Product name

  • Description

    Rabbit polyclonal to APLP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 262-491 of Human APLP2 (BC000373).

  • Positive control

    • Human fetal kidney and Human fetal liver lysate This antibody gave a positive result when used in the following methanol fixed cell line: DU145.

Properties

  • Form

    Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 98% PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab128603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 85 kDa (predicted molecular weight: 81, 85, 87 kDa).
IHC-P 1/100 - 1/500.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    May play a role in the regulation of hemostasis. The soluble form may have inhibitory properties towards coagulation factors. May interact with cellular G-protein signaling pathways. May bind to the DNA 5'-GTCACATG-3'(CDEI box). Inhibits trypsin, chymotrypsin, plasmin, factor XIA and plasma and glandular kallikrein.
  • Tissue specificity

    Expressed in placenta, brain, heart, lung, liver, kidney and endothelial tissues.
  • Sequence similarities

    Belongs to the APP family.
    Contains 1 BPTI/Kunitz inhibitor domain.
  • Post-translational
    modifications

    The BPTI/Kunitz inhibitor domain is O-glycosylated.
  • Cellular localization

    Cell membrane. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • amyloid beta (A4) precursor like protein 2 antibody
    • Amyloid like protein 2 antibody
    • Amyloid precursor protein homolog HSD 2 antibody
    • amyloid precursor protein homolog HSD2 antibody
    • Amyloid protein homolog antibody
    • Amyloid-like protein 2 antibody
    • APLP-2 antibody
    • Aplp2 antibody
    • APLP2_HUMAN antibody
    • APPH antibody
    • APPL2 antibody
    • CDEBP antibody
    • CDEI box binding protein antibody
    • CDEI box-binding protein antibody
    see all

Images

  • Anti-APLP2 antibody (ab128603) at 1/1000 dilution + Human fetal liver lysate

    Predicted band size: 81, 85, 87 kDa
    Observed band size: 85 kDa
    why is the actual band size different from the predicted?

  • ICC/IF image of ab128603 stained DU145 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab128603 at 5 ug/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab128603, at 1/100 dilution, staining APLP2 in paraffin-embedded Human fetal kidney by Immunohistochemistry

References

This product has been referenced in:

  • Gao L  et al. Role of APLP2 in the prognosis and clinicopathology of renal cell carcinoma. Oncol Lett 17:508-513 (2019). Read more (PubMed: 30655794) »
  • Paschkowsky S  et al. Alternative Processing of the Amyloid Precursor Protein Family by Rhomboid Protease RHBDL4. J Biol Chem 291:21903-21912 (2016). Read more (PubMed: 27563067) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

DISCOUNT CODE:

Ab109996: ***
Ab128603: ***

Expiration date: July 31, 2012

Eachcode will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview foreach APLP2 antibody in flow cytometryand include the correspondingcode in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Eachcode will be active once the correspondingAbreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreviews and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain and Spinal cord)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
30 µg
Specification
Brain and Spinal cord
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jun 06 2017

Question
Answer

Thank you for your call today and for your interest in our APLP2 antibodies. As we discussed, we do have a testing discount program if you'd like to use an antibody in an untested species or application. Please see below for more information about the program, and let me know if you have any questions.

Since ab109996 has not been tested in flow cytometry, I can offer a discount off a future purchase if you buy ab109996 now, test it inflow cytometry and submit feedback to us in the form of an Abreview. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and testab109996 in flow cytometry. I will then send a discount code. This code must be issued before purchasing ab109996 so please wait for my reply before ordering.

2. Purchase ab109996 either by phone, fax, or online (www.abcam.com).

3. Test it in flow cytometry.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab109996 turns out to be unsuitable for flow cytometry, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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