Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5938(2)] to APLP2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-APLP2 antibody [EPR5938(2)]
See all APLP2 primary antibodies
DescriptionRabbit monoclonal [EPR5938(2)] to APLP2
Specificityab140624 detects isoforms in some cell lines.
Tested applicationsSuitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human APLP2 aa 200-300. The exact sequence is proprietary.
Database link: Q06481
- Fetal kidney, HeLa, placenta, SH-SY5Y, fetal brain and 293T lysates; Human kidney tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab140624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/10000 - 1/50000. Detects a band of approximately 90-120 kDa (predicted molecular weight: 87 kDa).
ab140624 detects isoforms in some cell lines
FunctionMay play a role in the regulation of hemostasis. The soluble form may have inhibitory properties towards coagulation factors. May interact with cellular G-protein signaling pathways. May bind to the DNA 5'-GTCACATG-3'(CDEI box). Inhibits trypsin, chymotrypsin, plasmin, factor XIA and plasma and glandular kallikrein.
Tissue specificityExpressed in placenta, brain, heart, lung, liver, kidney and endothelial tissues.
Sequence similaritiesBelongs to the APP family.
Contains 1 BPTI/Kunitz inhibitor domain.
modificationsThe BPTI/Kunitz inhibitor domain is O-glycosylated.
Cellular localizationCell membrane. Nucleus.
- Information by UniProt
- amyloid beta (A4) precursor like protein 2 antibody
- Amyloid like protein 2 antibody
- Amyloid precursor protein homolog HSD 2 antibody
Anti-APLP2 antibody [EPR5938(2)] (ab140624) at 1/50000 dilution + LNCaP(Human prostate carcinoma epithelial cell)whole cell lysate at 20 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 87 kDa
Observed band size: 90-120 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Diluting/Blocking buffer: 5% NFDM /TBST
ab140624 detects isoforms in some cell lines and the bands are consistent with the literature (PMID: 16279945).
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling APLP2 with ab140624 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes : Anti-APLP2 antibody [EPR5938(2)] (ab140624) at 1/10000 dilution
Lane 1 : Fetal kidney tissue lysate
Lane 2 : HeLa cell lysate
Lane 3 : Placenta tissue lysate
Lane 4 : SH-SY5Y cell lysate
Lane 5 : Fetal brain tissue lysate
Lane 6 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 87 kDa
ab140624 has been referenced in 1 publication.
- Currinn H et al. APP controls the formation of PI(3,5)P(2) vesicles through its binding of the PIKfyve complex. Cell Mol Life Sci 73:393-408 (2016). PubMed: 26216398