Overview

  • Product name

    Anti-APLP2 antibody [EPR5938(2)]
    See all APLP2 primary antibodies
  • Description

    Rabbit monoclonal [EPR5938(2)] to APLP2
  • Host species

    Rabbit
  • Specificity

    ab140624 detects isoforms in some cell lines.
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human APLP2 aa 200-300. The exact sequence is proprietary.
    Database link: Q06481

  • Positive control

    • Fetal kidney, HeLa, placenta, SH-SY5Y, fetal brain and 293T lysates; Human kidney tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab140624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/10000 - 1/50000. Detects a band of approximately 90-120 kDa (predicted molecular weight: 87 kDa).

ab140624 detects isoforms in some cell lines

  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      May play a role in the regulation of hemostasis. The soluble form may have inhibitory properties towards coagulation factors. May interact with cellular G-protein signaling pathways. May bind to the DNA 5'-GTCACATG-3'(CDEI box). Inhibits trypsin, chymotrypsin, plasmin, factor XIA and plasma and glandular kallikrein.
    • Tissue specificity

      Expressed in placenta, brain, heart, lung, liver, kidney and endothelial tissues.
    • Sequence similarities

      Belongs to the APP family.
      Contains 1 BPTI/Kunitz inhibitor domain.
    • Post-translational
      modifications

      The BPTI/Kunitz inhibitor domain is O-glycosylated.
    • Cellular localization

      Cell membrane. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • amyloid beta (A4) precursor like protein 2 antibody
      • Amyloid like protein 2 antibody
      • Amyloid precursor protein homolog HSD 2 antibody
      • amyloid precursor protein homolog HSD2 antibody
      • Amyloid protein homolog antibody
      • Amyloid-like protein 2 antibody
      • APLP-2 antibody
      • Aplp2 antibody
      • APLP2_HUMAN antibody
      • APPH antibody
      • APPL2 antibody
      • CDEBP antibody
      • CDEI box binding protein antibody
      • CDEI box-binding protein antibody
      see all

    Images

    • Anti-APLP2 antibody [EPR5938(2)] (ab140624) at 1/50000 dilution + LNCaP(Human prostate carcinoma epithelial cell)whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 87 kDa
      Observed band size: 90-120 kDa
      why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      Diluting/Blocking buffer: 5% NFDM /TBST

      ab140624 detects isoforms in some cell lines and the bands are consistent with the literature (PMID: 16279945). 

       

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling APLP2 with ab140624 at 1/100 dilution.
    • All lanes : Anti-APLP2 antibody [EPR5938(2)] (ab140624) at 1/10000 dilution

      Lane 1 : Fetal kidney tissue lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Placenta tissue lysate
      Lane 4 : SH-SY5Y cell lysate
      Lane 5 : Fetal brain tissue lysate
      Lane 6 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 87 kDa

    References

    This product has been referenced in:

    • Currinn H  et al. APP controls the formation of PI(3,5)P(2) vesicles through its binding of the PIKfyve complex. Cell Mol Life Sci 73:393-408 (2016). Read more (PubMed: 26216398) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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