Overview

  • Product name

  • Description

    Goat polyclonal to Apo-D
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cynomolgus monkey
  • Immunogen

    Synthetic peptide corresponding to Human Apo-D aa 48-61.
    Sequence:

    EIEKIPTTFENGRC


    Database link: P05090

  • Positive control

    • WB: Human liver lysate. IHC-P: Human kidney.
  • General notes

    Previously labelled as Apolipoprotein D. 

Properties

Applications

Our Abpromise guarantee covers the use of ab223252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 21.3 kDa).
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    APOD occurs in the macromolecular complex with lecithin-cholesterol acyltransferase. It is probably involved in the transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts.
  • Tissue specificity

    Expressed in liver, intestine, pancreas, kidney, placenta, adrenal, spleen, fetal brain tissue and tears.
  • Sequence similarities

    Belongs to the calycin superfamily. Lipocalin family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • APO D antibody
    • Apo-D antibody
    • ApoD antibody
    • APOD protein antibody
    • APOD_HUMAN antibody
    • Apolipoprotein D antibody
    • ApolipoproteinD antibody
    see all

Images

  • Paraffin embedded sections of human adrenal gland were stained for Apo-D with ab223252 at a concentration of 5 μg/ml in Immunohistochemical analysis.

  • Anti-PKC beta 1 antibody [EPR18512] (Alexa Fluor® 488) (ab223452) at 0.3 µg/ml + Human liver lysate at 35 µg

    Predicted band size: 21.3 kDa
    Observed band size: 25 kDa
    why is the actual band size different from the predicted?



    Primary incubation: 1 hour

  • Paraffin embedded sections of human kidney were stained for Apo-D with ab223252 at a concentration of 5 μg/ml in Immunohistochemical analysis.

References

ab223252 has not yet been referenced specifically in any publications.

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