RecombinantRabMAb

Recombinant Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)

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Western blot - Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)
  • Western blot - Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)
  • Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2916] to Apo-D - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Apo-D antibody [EPR2916] - BSA and Azide free
    See all Apo-D primary antibodies

  • Description

    Rabbit monoclonal [EPR2916] to Apo-D - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Apo-D aa 1-100. The exact sequence is proprietary.

  • General notes

    ab247602 is the carrier-free version of ab108191 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab247602 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab247602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
WB Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.

Target

  • Function

    APOD occurs in the macromolecular complex with lecithin-cholesterol acyltransferase. It is probably involved in the transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts.

  • Tissue specificity

    Expressed in liver, intestine, pancreas, kidney, placenta, adrenal, spleen, fetal brain tissue and tears.

  • Sequence similarities

    Belongs to the calycin superfamily. Lipocalin family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • APO D antibody
      • Apo-D antibody
      • ApoD antibody
      • APOD protein antibody
      • APOD_HUMAN antibody
      • Apolipoprotein D antibody
      • ApolipoproteinD antibody
      see all

    Images

    • Western blot - Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)
      Western blot - Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)
      Anti-Apo-D antibody [EPR2916] (ab108191) at 1/2000 dilution (purified) + human plasma at 10 µg

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

      Predicted band size: 21 kDa
      Observed band size: 30 kDa
      why is the actual band size different from the predicted?



      This data was developed using ab108191, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)
      This data was developed using ab108191, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin embedded human liver with purified ab108191 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Western blot - Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)
      Western blot - Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)
      Anti-Apo-D antibody [EPR2916] (ab108191) at 1/1000 dilution (unpurified) + Human plasma at 10 µg

      Predicted band size: 21 kDa



      This data was developed using ab108191, the same antibody clone in a different buffer formulation.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)
      This data was developed using ab108191, the same antibody clone in a different buffer formulation.Immunohistochemical staining of Apo-D in paraffin embedded human kidney tissue, using unpurified ab108191 at a 1/100 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)
      This data was developed using ab108191, the same antibody clone in a different buffer formulation.Immunohistochemical staining of Apo-D in paraffin embedded Human liver tissue, using unpurified ab108191 at a 1/100 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    • Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)
      Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab247602? Please let us know so that we can cite the reference in this datasheet.

    ab247602 has not yet been referenced specifically in any publications.

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