Overview

  • Product name
    Anti-Apo-H antibody [EPR2898(2)]
    See all Apo-H primary antibodies
  • Description
    Rabbit monoclonal [EPR2898(2)] to Apo-H
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, ICC, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human Apo-H aa 50-150. The exact sequence is proprietary.

  • Positive control
    • Human fetal liver, Human plasma, human fetal kidney, and HDL lysates, HepG2 cells
  • General notes

     

     

    Previously labelled as Apolipoprotein H. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 38 kDa.
IP 1/10 - 1/100.
ICC 1/100 - 1/250.
ICC/IF 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function
      Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.
    • Tissue specificity
      Expressed by the liver and secreted in plasma.
    • Sequence similarities
      Contains 4 Sushi (CCP/SCR) domains.
    • Post-translational
      modifications
      N- and O-glycosylated. PubMed:6587378 also reports glycosylation on 'Asn-188' for their allele.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activated protein C binding protein antibody
      • Activated protein C-binding protein antibody
      • Anticardiolipin cofactor antibody
      • APC inhibitor antibody
      • Apo-H antibody
      • APOH antibody
      • APOH_HUMAN antibody
      • apolipoprotein H (beta-2-glycoprotein I) antibody
      • Apolipoprotein H antibody
      • B2G1 antibody
      • B2GP1 antibody
      • B2GPI antibody
      • Beta 2 glycoprotein I antibody
      • Beta 2 glycoprotein I precursor antibody
      • Beta(2)GPI antibody
      • Beta-2-glycoprotein 1 antibody
      • Beta-2-glycoprotein I antibody
      • BG antibody
      • Glycoprotein 1, beta-2 antibody
      • Glycoprotein I, beta-2 antibody
      • OTTMUSP00000003033 antibody
      see all

    Images

    • Immunofluorescent staining of HepG2 cells using ab108348 at a dilution of 1/100.
    • All lanes : Anti-Apo-H antibody [EPR2898(2)] (ab108348) at 1/10000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : Human plasma lysate
      Lane 3 : Human fetal kidney lysate
      Lane 4 : HDL lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 38 kDa

    References

    This product has been referenced in:
    • Sá E Cunha C  et al. Plasmodium berghei EXP-1 interacts with host Apolipoprotein H duringPlasmodiumliver-stage development. Proc Natl Acad Sci U S A 114:E1138-E1147 (2017). Read more (PubMed: 28137845) »
    • Yao Y  et al. Screening and identification of potential predictive biomarkers for Down's syndrome from second trimester maternal serum. Expert Rev Proteomics 12:97-107 (2015). WB ; Human . Read more (PubMed: 25434962) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for contacting us. I am sorry to hear you have been experiencing some problems. We have received no other complaints about this product, so in this case it is highly likely that some changes to the protocol will help. Not all antibodies will work under standard conditions, therefore some individual optimisation may be required to help clean up the signal. - Do not dilute the antibody in PBS prior to storage. Simply aliquot into smaller volumes and freeze at -20°C. - Dilute the primary antibody 1:10,000 - 1:50,000. This should reduce the background staining. As this antibody is whole antiserum it isn't unusual to see some background. - Are you using BSA or milk for blocking? BSA can sometimes reduce the background. - How is the primary antibody incubated. I would suggest overnight at 4°C I look forward to your reply.

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    Answer

    Thank you for your reply. Please could you provide some further information about how the antibody has been used? I have included some questions below. Order Details Antibody code: Problem Choose: Non-specific band Multiple bands No signal or weak signal High background Lot number Purchase order number or preferably Abcam order number: General Information Antibody storage conditions (temperature/reconstitution etc) Description of the problem (high background, wrong band size, more bands, no band etc.) Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.) Sample preparation (Buffer/Protease inhibitors/Heating sample etc.) Amount of protein loaded Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.) Transfer and blocking conditions (Buffer/time period, Blocking agent etc.) Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step) Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step) Detection method (ECL, ECLPlus etc.) Positive and negative controls used (please specify) Optimization attempts (problem solving) How many times have you tried the Western? Have you run a "No Primary" control? Yes No Do you obtain the same results every time? Yes No e.g. are the background bands always in the same place? What steps have you altered? Additional Notes: Image: We would appreciate if you are able to provide an image (including molecular weight markers) which would help us to asess the results.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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