Anti-Apo-M antibody [8F12C6B8] (ab66379)
Key features and details
- Mouse monoclonal [8F12C6B8] to Apo-M
- Suitable for: Flow Cyt, WB, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-Apo-M antibody [8F12C6B8]
See all Apo-M primary antibodies -
Description
Mouse monoclonal [8F12C6B8] to Apo-M -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human, African green monkey -
Immunogen
Recombinant full length protein corresponding to Apo-M.
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Positive control
- ICC/IF: L-02 cells; COS-7 cells WB: Human serum
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
8F12C6B8 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab66379 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB |
1/500 - 1/2000. Predicted molecular weight: 21 kDa.
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ICC/IF |
1/200 - 1/1000.
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Notes |
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Flow Cyt
Use 2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
1/500 - 1/2000. Predicted molecular weight: 21 kDa. |
ICC/IF
1/200 - 1/1000. |
Target
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Function
Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid. -
Tissue specificity
Plasma protein. Expressed in liver and kidney. -
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. Highly divergent. -
Cellular localization
Secreted. Present in high density lipoprotein (HDL) and to a lesser extent in triglyceride-rich lipoproteins (TGRLP) and low density lipoproteins. - Information by UniProt
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Database links
- Entrez Gene: 55937 Human
- Omim: 606907 Human
- SwissProt: O95445 Human
- Unigene: 534468 Human
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Alternative names
- Apo M antibody
- Apo-M antibody
- Apolipoprotein M antibody
see all
Images
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All lanes : Anti-Apo-M antibody [8F12C6B8] (ab66379) at 1/500 dilution
All lanes : human serum
Predicted band size: 21 kDa -
Immunofluorescnece analysis of COS-7 cells using ab66739 at a dilution of 1/200 - 1/1000, showing cytomembrane localization.
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Immunofluorescnece analysis of L-02 cells using ab66739 at a dilution of 1/200 - 1/1000, showing cytomembrane localization.
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Overlay histogram showing HepG2 cells stained with ab66379 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab66379, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Protocols
Datasheets and documents
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Datasheet download
References (4)
ab66379 has been referenced in 4 publications.
- Dai Y et al. A Multivariate Diagnostic Model Based on Urinary EpCAM-CD9-Positive Extracellular Vesicles for Prostate Cancer Diagnosis. Front Oncol 11:777684 (2021). PubMed: 34900726
- Yang L et al. Expression of apolipoprotein M and its association with adiponectin in an obese mouse model. Exp Ther Med 18:1685-1692 (2019). PubMed: 31410126
- Yang L et al. Niacin regulates apolipoprotein M expression via liver X receptor-a. Mol Med Rep 20:3285-3291 (2019). PubMed: 31432166
- Liu M et al. Hepatic apolipoprotein M (apoM) overexpression stimulates formation of larger apoM/sphingosine 1-phosphate-enriched plasma high density lipoprotein. J Biol Chem 289:2801-14 (2014). WB ; Mouse, Human . PubMed: 24318881