Overview

  • Product name

    Anti-Apo-M antibody [8F12C6B8]
    See all Apo-M primary antibodies
  • Description

    Mouse monoclonal [8F12C6B8] to Apo-M
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, WB, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Apo-M.

  • Positive control

    • ICC/IF: L-02 cells; COS-7 cells WB: Human serum

Properties

Applications

Our Abpromise guarantee covers the use of ab66379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB 1/500 - 1/2000. Predicted molecular weight: 21 kDa.
ELISA 1/10000.
ICC/IF 1/200 - 1/1000.

Target

  • Function

    Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.
  • Tissue specificity

    Plasma protein. Expressed in liver and kidney.
  • Sequence similarities

    Belongs to the calycin superfamily. Lipocalin family. Highly divergent.
  • Cellular localization

    Secreted. Present in high density lipoprotein (HDL) and to a lesser extent in triglyceride-rich lipoproteins (TGRLP) and low density lipoproteins.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apo M antibody
    • Apo-M antibody
    • Apolipoprotein M antibody
    • ApoM antibody
    • APOM_HUMAN antibody
    • G3A antibody
    • HSPC336 antibody
    • MGC22400 antibody
    • NG20 antibody
    • NG20 like protein antibody
    • Protein G3a antibody
    see all

Images

  • All lanes : Anti-Apo-M antibody [8F12C6B8] (ab66379) at 1/500 dilution

    All lanes : human serum

    Predicted band size: 21 kDa

  • Immunofluorescnece analysis of COS-7 cells using ab66739 at a dilution of 1/200 - 1/1000, showing cytomembrane localization.
  • Immunofluorescnece analysis of L-02 cells using ab66739 at a dilution of 1/200 - 1/1000, showing cytomembrane localization.
  • Overlay histogram showing HepG2 cells stained with ab66379 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab66379, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:

  • Liu M  et al. Hepatic apolipoprotein M (apoM) overexpression stimulates formation of larger apoM/sphingosine 1-phosphate-enriched plasma high density lipoprotein. J Biol Chem 289:2801-14 (2014). WB ; Mouse, Human . Read more (PubMed: 24318881) »
See 1 Publication for this product

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