Overview

  • Product name

  • Description

    Goat polyclonal to Apo-M
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Mouse Apo-M aa 108-120 (internal sequence).
    Sequence:

    C-KGNMELRTEGRPD


    Database link: NP_061286.1

  • Positive control

    • WB: Human placenta lysate. IHC-P: Human liver tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab85695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 21 kDa).

The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xu and Dahlbäck, J Biol Chem. 1999 Oct 29;274(44):31286-90.; PMID: 10531326).

IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.
  • Tissue specificity

    Plasma protein. Expressed in liver and kidney.
  • Sequence similarities

    Belongs to the calycin superfamily. Lipocalin family. Highly divergent.
  • Cellular localization

    Secreted. Present in high density lipoprotein (HDL) and to a lesser extent in triglyceride-rich lipoproteins (TGRLP) and low density lipoproteins.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apo M antibody
    • Apo-M antibody
    • Apolipoprotein M antibody
    • ApoM antibody
    • APOM_HUMAN antibody
    • G3A antibody
    • HSPC336 antibody
    • MGC22400 antibody
    • NG20 antibody
    • NG20 like protein antibody
    • Protein G3a antibody
    see all

Images

  • Anti-Apo-M antibody (ab85695) at 0.5 µg/ml + Human placenta lysate (in RIPA buffer) at 35 µg

    Predicted band size: 21 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected by chemiluminescence.
  • ab85695 at 3.75 µg/ml staining of paraffin embedded human liver tissue. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References

ab85695 has not yet been referenced specifically in any publications.

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