Recombinant Anti-Apo-M antibody [EPR2904] (ab91656)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2904] to Apo-M
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Apo-M antibody [EPR2904]
See all Apo-M primary antibodies -
Description
Rabbit monoclonal [EPR2904] to Apo-M -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Apo-M aa 50-150. The exact sequence is proprietary.
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Positive control
- WB: Human, rat, and mouse plasma lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2904 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab91656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/2000. Predicted molecular weight: 21 kDa.
For unpurified use at 1/2000 - 1/10000. |
Notes |
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WB
1/2000. Predicted molecular weight: 21 kDa. For unpurified use at 1/2000 - 1/10000. |
Target
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Function
Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid. -
Tissue specificity
Plasma protein. Expressed in liver and kidney. -
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. Highly divergent. -
Cellular localization
Secreted. Present in high density lipoprotein (HDL) and to a lesser extent in triglyceride-rich lipoproteins (TGRLP) and low density lipoproteins. - Information by UniProt
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Database links
- Entrez Gene: 55937 Human
- Entrez Gene: 55938 Mouse
- Entrez Gene: 55939 Rat
- Omim: 606907 Human
- SwissProt: O95445 Human
- SwissProt: Q9Z1R3 Mouse
- SwissProt: P14630 Rat
- Unigene: 534468 Human
see all -
Alternative names
- Apo M antibody
- Apo-M antibody
- Apolipoprotein M antibody
see all
Images
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All lanes : Anti-Apo-M antibody [EPR2904] (ab91656) at 1/2000 dilution (purified)
Lane 1 : Human plasma lysate
Lane 2 : Mouse plasma lysate
Lane 3 : Rat plasma lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 21 kDa
Observed band size: 18,23 kDa why is the actual band size different from the predicted?The molecular weights observed are consistent with what have been described in the literature (PMID: 15102887, 16682745, 10531326)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab91656 has been referenced in 4 publications.
- Del Gaudio I et al. Circulating cord blood HDL-S1P complex preserves the integrity of the feto-placental vasculature. Biochim Biophys Acta Mol Cell Biol Lipids 1865:158632 (2020). PubMed: 31954174
- Obinata H et al. Identification of ApoA4 as a sphingosine 1-phosphate chaperone in ApoM- and albumin-deficient mice. J Lipid Res 60:1912-1921 (2019). PubMed: 31462513
- Burg N et al. Sphingosine 1-Phosphate Receptor 1 Signaling Maintains Endothelial Cell Barrier Function and Protects Against Immune Complex-Induced Vascular Injury. Arthritis Rheumatol 70:1879-1889 (2018). PubMed: 29781582
- Bosteen MH et al. Protein unfolding allows use of commercial antibodies in an apolipoprotein M sandwich ELISA. J Lipid Res 56:754-9 (2015). PubMed: 25561460