Recombinant Anti-Apo-M antibody [EPR2904] - BSA and Azide free (ab239902)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2904] to Apo-M - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Apo-M antibody [EPR2904] - BSA and Azide free
See all Apo-M primary antibodies -
Description
Rabbit monoclonal [EPR2904] to Apo-M - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human, rat, and mouse plasma lysates.
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General notes
ab239902 is the carrier-free version of ab91656.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2904 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Immunohistochemistry kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab239902 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa. |
Target
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Function
Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid. -
Tissue specificity
Plasma protein. Expressed in liver and kidney. -
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. Highly divergent. -
Cellular localization
Secreted. Present in high density lipoprotein (HDL) and to a lesser extent in triglyceride-rich lipoproteins (TGRLP) and low density lipoproteins. - Information by UniProt
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Database links
- Entrez Gene: 55937 Human
- Entrez Gene: 55938 Mouse
- Entrez Gene: 55939 Rat
- Omim: 606907 Human
- SwissProt: O95445 Human
- SwissProt: Q9Z1R3 Mouse
- SwissProt: P14630 Rat
- Unigene: 534468 Human
see all -
Alternative names
- Apo M antibody
- Apo-M antibody
- Apolipoprotein M antibody
see all
Images
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All lanes : Anti-Apo-M antibody [EPR2904] (ab91656) at 1/2000 dilution (purified)
Lane 1 : Human plasma lysate
Lane 2 : Mouse plasma lysate
Lane 3 : Rat plasma lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 21 kDa
Observed band size: 18,23 kDa why is the actual band size different from the predicted?The molecular weights observed are consistent with what have been described in the literature (PMID: 15102887, 16682745, 10531326).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab91656).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab239902 has not yet been referenced specifically in any publications.