Overview

  • Product name

  • Description

    Rabbit polyclonal to APOA1BP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal sequence of human APOA1BP

  • Positive control

    • Extracts from HeLa cells. A549 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 32 kDa.
ELISA 1/40000.
ICC/IF 1/500 - 1/1000.

Target

  • Relevance

    APOA1BP interacts with apolipoprotein A-I (apoA-I), the major apolipoprotein of high-density lipoproteins (HDLs). It is secreted into some bodily fluids, and its synthesis and secretion are stimulated in vitro by incubating cells with apoA-I.
  • Cellular localization

    Mitochondrion. Secreted. Note: In sperm, secretion gradually increases during capacitation
  • Database links

  • Alternative names

    • AI BP antibody
    • AIBP antibody
    • ApoA I binding protein antibody
    • Apolipoprotein A I binding protein antibody
    • MGC119143 antibody
    • MGC119144 antibody
    • MGC119145 antibody
    • NAD(P)H hydrate epimerase antibody
    • NAD(P)HX epimerase antibody
    • YjeF N terminal domain containing protein 1 antibody
    • yjeF_N1 antibody
    • YJEFN1 antibody
    see all

Images

  • All lanes : Anti-APOA1BP antibody (ab75114) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells
    Lane 2 : Extracts from HeLa cells with immunizing peptide at 5 µg

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 32 kDa
    Observed band size: 29 kDa
    why is the actual band size different from the predicted?

  • Immunofluorescence analysis of APOA1BP in A549 cells, using ab75114, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

References

This product has been referenced in:

  • Choi SH  et al. AIBP augments cholesterol efflux from alveolar macrophages to surfactant and reduces acute lung inflammation. JCI Insight 3:N/A (2018). Read more (PubMed: 30135304) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Multiple cell lines)
Gel Running Conditions
Reduced Denaturing
Loading amount
70 µg
Specification
Multiple cell lines
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jun 15 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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