Anti-APOA4/Apo-AIV antibody (ab231660)
Key features and details
- Rabbit polyclonal to APOA4/Apo-AIV
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-APOA4/Apo-AIV antibody
See all APOA4/Apo-AIV primary antibodies -
Description
Rabbit polyclonal to APOA4/Apo-AIV -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment corresponding to Mouse APOA4/Apo-AIV aa 250 to the C-terminus. (N-terminal His-tag and S-tag; Expressed in E.coli).
Database link: P06728 -
Positive control
- WB: Mouse lung lysate; Recombinant mouse APOA4/Apo-AIV protein. IHC-P: Mouse intestine tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab231660 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231660 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 45 kDa.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 45 kDa. |
Target
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Relevance
APOA4 (apolipoprotein A-IV) is a component of HDL and chylomicrons. Its primary site of synthesis is the intestine, in association with lymph chylomicron particles. Although its precise function is not known, APOA4 is a potent activator of lecithin-cholesterol acyltransferase (LCAT) in vitro. In rodents, Apo A-IV inhibits gastric emptying and serves as a satiety factor whose synthesis and secretion are increased by the ingestion of dietary fat. It also possesses anti-inflammatory and antiatherogenic properties -
Cellular localization
Secreted -
Database links
- Entrez Gene: 11808 Mouse
- SwissProt: P06728 Mouse
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Alternative names
- Apo AIV antibody
- APOA 4 antibody
- ApoA IV antibody
see all
Images
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Formalin-fixed, paraffin-embedded mouse intestine tissue stained for APOA4/Apo-AIV using ab231660 at 10 µg/ml in immunohistochemical analysis.
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Anti-APOA4/Apo-AIV antibody (ab231660) at 2 µg/ml + Mouse lung lysate
Predicted band size: 45 kDa -
Anti-APOA4/Apo-AIV antibody (ab231660) at 2 µg/ml + Recombinant mouse APOA4/Apo-AIV protein
Predicted band size: 45 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab231660 has been referenced in 1 publication.
- Aliluev A et al. Diet-induced alteration of intestinal stem cell function underlies obesity and prediabetes in mice. Nat Metab 3:1202-1216 (2021). PubMed: 34552271