Overview

  • Product name

    Anti-APOA4/Apo-AIV antibody
    See all APOA4/Apo-AIV primary antibodies
  • Description

    Rabbit polyclonal to APOA4/Apo-AIV
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Monkey, Baboon
  • Immunogen

    Synthetic peptide corresponding to Human APOA4/Apo-AIV aa 300 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab92681)

  • Positive control

    • This antibody gave a positive signal in human plasma total protein tissue lysate.
  • General notes

     This product was previously labelled as APOA4

     

Applications

Our Abpromise guarantee covers the use of ab82355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      APOA4 (apolipoprotein A-IV) is a component of HDL and chylomicrons. Its primary site of synthesis is the intestine, in association with lymph chylomicron particles. Although its precise function is not known, APOA4 is a potent activator of lecithin-cholesterol acyltransferase (LCAT) in vitro. In rodents, Apo A-IV inhibits gastric emptying and serves as a satiety factor whose synthesis and secretion are increased by the ingestion of dietary fat. It also possesses anti-inflammatory and antiatherogenic properties
    • Cellular localization

      Secreted
    • Database links

    • Alternative names

      • Apo AIV antibody
      • APOA 4 antibody
      • ApoA IV antibody
      • APOA4 antibody
      • Apolipoprotein A IV antibody
      • Apolipoprotein A4 antibody
      • MGC142154 antibody
      • MGC142156 antibody
      see all

    Images

    • Anti-APOA4/Apo-AIV antibody (ab82355) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 45 kDa
      Observed band size: 45 kDa


      Exposure time: 8 minutes

    References

    ab82355 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Answer

    Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

    Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab82355.

    I would suggest switching to a BSA block for 1-2 hours at room temperature. I would also increase your washes (both after primary and after secondary) to maybe 15 min each. Usually your protocol would work well but in cases like this where the antibody is showing such high background with almost a "ghost band" effect this can help improve your image.


    Should the suggestions not improve the results, please do let me know.

    In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

    I hope this information is helpful, and I thank you for your cooperation.

    Read More

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