Anti-APOA4/Apo-AIV antibody [CL0468] (ab211867)
Key features and details
- Mouse monoclonal [CL0468] to APOA4/Apo-AIV
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-APOA4/Apo-AIV antibody [CL0468]
See all APOA4/Apo-AIV primary antibodies -
Description
Mouse monoclonal [CL0468] to APOA4/Apo-AIV -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cynomolgus monkey -
Immunogen
Recombinant fragment corresponding to Human APOA4/Apo-AIV aa 200 to the C-terminus.
Database link: P06727 -
Epitope
Binds to an epitope located within the peptide sequence GHLSFLEKDL as determined by overlapping synthetic peptides. -
Positive control
- IHC-P: Human duodenum, kidney, rectum, liver and fallopian tube tissues; WB: Human plasma cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CL0468 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab211867 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 45 kDa.
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Notes |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/500 - 1/1000. Predicted molecular weight: 45 kDa. |
Target
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Relevance
APOA4 (apolipoprotein A-IV) is a component of HDL and chylomicrons. Its primary site of synthesis is the intestine, in association with lymph chylomicron particles. Although its precise function is not known, APOA4 is a potent activator of lecithin-cholesterol acyltransferase (LCAT) in vitro. In rodents, Apo A-IV inhibits gastric emptying and serves as a satiety factor whose synthesis and secretion are increased by the ingestion of dietary fat. It also possesses anti-inflammatory and antiatherogenic properties -
Cellular localization
Secreted -
Database links
- Entrez Gene: 337 Human
- Omim: 107690 Human
- SwissProt: P33621 Cynomolgus monkey
- SwissProt: P06727 Human
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Alternative names
- Apo AIV antibody
- APOA 4 antibody
- ApoA IV antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human fallopian tube tissue labeling APOA4/Apo-AIV with ab211867 at 1/200 dilution.
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Anti-APOA4/Apo-AIV antibody [CL0468] (ab211867) at 1/500 dilution + Human plasma
Predicted band size: 45 kDa -
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling APOA4/Apo-AIV with ab211867 at 1/200 dilution.
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Negative control: Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling APOA4/Apo-AIV with ab211867 at 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human rectum tissue labeling APOA4/Apo-AIV with ab211867 at 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling APOA4/Apo-AIV with ab211867 at 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human duodenum tissue labeling APOA4/Apo-AIV with ab211867 at 1/200 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab211867 has not yet been referenced specifically in any publications.