Recombinant
RabMAb

Recombinant Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)

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Overview

  • Product name

    Anti-APOBEC2 antibody [EPR11285(B)]
    See all APOBEC2 primary antibodies

  • Description

    Rabbit monoclonal [EPR11285(B)] to APOBEC2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human APOBEC2 (Uniprot: Q9Y235)

  • Positive control

    • fetal muscle and fetal heart lysates; rat muscle lysate; mouse muscle lysate; skeletal muscle tissue.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab157205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 26 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Relevance

      APOBEC2 belongs to the cytidine and deoxycytidylate deaminase family. It is probable C to U editing enzyme whose physiological substrate is not yet known. It does not display detectable apoB mRNA editing and has a low intrinsic cytidine deaminase activity.

    • Database links

      see all

    • Alternative names

      • APOBEC1-LIKE antibody
      • Apolipoprotein B mRNA editing enzyme catalytic polypeptide 2 antibody
      • Apolipoprotein B mRNA editing enzyme catalytic polypeptide like 2 antibody
      • ARCD1 antibody
      • ARP1 antibody
      • MGC128604 antibody
      • probable C->U-editing enzyme APOBEC-2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skeletal muscle tissue, labeling APOBEC2 using ab157205 at a 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)
      Western blot - Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)
      All lanes : Anti-APOBEC2 antibody [EPR11285(B)] (ab157205) at 1/10000 dilution

      Lane 1 : fetal Human muscle lysate
      Lane 2 : rat muscle lysate
      Lane 3 : mouse muscle lysate
      Lane 4 : fetal Huamn heart lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 26 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)

      ab157205 showing +ve staining in Human heart.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)

      ab157205 showing -ve staining in Human normal uterus.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)

      ab157205 showing -ve staining in Human normal brain.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    ab157205 has not yet been referenced specifically in any publications.

    Publishing research using ab157205? Please let us know so that we can cite the reference in this datasheet.

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