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  1. Link

    apobec2-antibody-epr11285b-ab157205.pdf

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Epigenetics and Nuclear Signaling Chromatin Binding Proteins DNA / RNA binding
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RecombinantRabMAb

Recombinant Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)
  • Western blot - Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)
  • Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11285(B)] to APOBEC2
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-APOBEC2 antibody [EPR11285(B)]
    See all APOBEC2 primary antibodies
  • Description

    Rabbit monoclonal [EPR11285(B)] to APOBEC2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human APOBEC2 (Uniprot: Q9Y235)

  • Positive control

    • fetal muscle and fetal heart lysates; rat muscle lysate; mouse muscle lysate; skeletal muscle tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11285(B)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Deamination

Associated products

  • Alternative Versions

    • Anti-APOBEC2 antibody [EPR11285(B)] - BSA and Azide free (ab249308)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Mouse skeletal muscle tissue lysate - total protein (ab29711)

Applications

Our Abpromise guarantee covers the use of ab157205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 26 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Relevance

      APOBEC2 belongs to the cytidine and deoxycytidylate deaminase family. It is probable C to U editing enzyme whose physiological substrate is not yet known. It does not display detectable apoB mRNA editing and has a low intrinsic cytidine deaminase activity.
    • Database links

      • Entrez Gene: 10930 Human
      • Entrez Gene: 11811 Mouse
      • Entrez Gene: 301226 Rat
      • Omim: 604797 Human
      • SwissProt: Q9Y235 Human
      • SwissProt: Q9WV35 Mouse
      • Unigene: 555915 Human
      • Unigene: 281793 Mouse
      • Unigene: 163040 Rat
      see all
    • Alternative names

      • APOBEC1-LIKE antibody
      • Apolipoprotein B mRNA editing enzyme catalytic polypeptide 2 antibody
      • Apolipoprotein B mRNA editing enzyme catalytic polypeptide like 2 antibody
      • ARCD1 antibody
      • ARP1 antibody
      • MGC128604 antibody
      • probable C->U-editing enzyme APOBEC-2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skeletal muscle tissue, labeling APOBEC2 using ab157205 at a 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)
      Western blot - Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)
      All lanes : Anti-APOBEC2 antibody [EPR11285(B)] (ab157205) at 1/10000 dilution

      Lane 1 : fetal Human muscle lysate
      Lane 2 : rat muscle lysate
      Lane 3 : mouse muscle lysate
      Lane 4 : fetal human heart lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 26 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)

      ab157205 showing +ve staining in Human heart.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)

      ab157205 showing -ve staining in Human normal uterus.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)

      ab157205 showing -ve staining in Human normal brain.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)
      Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab157205? Please let us know so that we can cite the reference in this datasheet.

    ab157205 has been referenced in 1 publication.

    • Lin X  et al. Simiao Decoction Alleviates Gouty Arthritis by Modulating Proinflammatory Cytokines and the Gut Ecosystem. Front Pharmacol 11:955 (2020). PubMed: 32670069

    Customer reviews and Q&As

    Show All Reviews Q&A
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    There are currently no Customer reviews or Questions for ab157205.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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