Recombinant Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11285(B)] to APOBEC2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-APOBEC2 antibody [EPR11285(B)]
See all APOBEC2 primary antibodies -
Description
Rabbit monoclonal [EPR11285(B)] to APOBEC2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human APOBEC2 (Uniprot: Q9Y235)
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Positive control
- fetal muscle and fetal heart lysates; rat muscle lysate; mouse muscle lysate; skeletal muscle tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11285(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab157205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/10000 - 1/50000. Predicted molecular weight: 26 kDa. | |
IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
APOBEC2 belongs to the cytidine and deoxycytidylate deaminase family. It is probable C to U editing enzyme whose physiological substrate is not yet known. It does not display detectable apoB mRNA editing and has a low intrinsic cytidine deaminase activity. -
Database links
- Entrez Gene: 10930 Human
- Entrez Gene: 11811 Mouse
- Entrez Gene: 301226 Rat
- Omim: 604797 Human
- SwissProt: Q9Y235 Human
- SwissProt: Q9WV35 Mouse
- Unigene: 555915 Human
- Unigene: 281793 Mouse
see all -
Alternative names
- APOBEC1-LIKE antibody
- Apolipoprotein B mRNA editing enzyme catalytic polypeptide 2 antibody
- Apolipoprotein B mRNA editing enzyme catalytic polypeptide like 2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skeletal muscle tissue, labeling APOBEC2 using ab157205 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-APOBEC2 antibody [EPR11285(B)] (ab157205) at 1/10000 dilution
Lane 1 : fetal Human muscle lysate
Lane 2 : rat muscle lysate
Lane 3 : mouse muscle lysate
Lane 4 : fetal human heart lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 26 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)
ab157205 showing +ve staining in Human heart.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)
ab157205 showing -ve staining in Human normal uterus.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] (ab157205)
ab157205 showing -ve staining in Human normal brain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
References (1)
ab157205 has been referenced in 1 publication.
- Lin X et al. Simiao Decoction Alleviates Gouty Arthritis by Modulating Proinflammatory Cytokines and the Gut Ecosystem. Front Pharmacol 11:955 (2020). PubMed: 32670069