Recombinant Anti-APOBEC2 antibody [EPR11286(B)] (ab170859)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (ab170859)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11286(B)] to APOBEC2
Suitable for: IHC-P, WB
Reacts with: Human

Product name

Anti-APOBEC2 antibody [EPR11286(B)]
See all APOBEC2 primary antibodies
Description

Rabbit monoclonal [EPR11286(B)] to APOBEC2
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human APOBEC2 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9Y235
Positive control
- Human heart and skeletal muscle tissues; Human fetal muscle and fetal heart lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11286(B)
Isotype

IgG
Research areas

Alternative Versions
- Anti-APOBEC2 antibody [EPR11286(B)] - BSA and Azide free (ab249542)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab170859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application	Species
ICC	Human
IHC-P	Human
WB	Human
All applications	Mouse Rat

Application	Abreviews	Notes
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB		1/10000 - 1/50000. Predicted molecular weight: 26 kDa.

Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/10000 - 1/50000. Predicted molecular weight: 26 kDa.

Application notes

Is unsuitable for ICC/IF or IP.

Relevance

APOBEC2 belongs to the cytidine and deoxycytidylate deaminase family. It is probable C to U editing enzyme whose physiological substrate is not yet known. It does not display detectable apoB mRNA editing and has a low intrinsic cytidine deaminase activity.
Database links
- Entrez Gene: 10930 Human
- Entrez Gene: 11811 Mouse
- Entrez Gene: 301226 Rat
- Omim: 604797 Human
- SwissProt: Q9Y235 Human
- SwissProt: Q9WV35 Mouse
- Unigene: 555915 Human
- Unigene: 281793 Mouse
- Unigene: 163040 Rat
see all
Alternative names
- APOBEC1-LIKE antibody
- Apolipoprotein B mRNA editing enzyme catalytic polypeptide 2 antibody
- Apolipoprotein B mRNA editing enzyme catalytic polypeptide like 2 antibody
- ARCD1 antibody
- ARP1 antibody
- MGC128604 antibody
- probable C->U-editing enzyme APOBEC-2 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (ab170859)

Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human skeletal muscle tissue labeling APOBEC2 with ab170859 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (ab170859)

Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human heart tissue labeling APOBEC2 with ab170859 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (ab170859)

ab170859 showing -ve staining in Human thyroid gland carcinoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (ab170859)

ab170859 showing -ve staining in Human normal liver tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (ab170859)

ab170859 showing -ve staining in Human normal kidney tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (ab170859)

ab170859 showing -ve staining in Human cervical carcinoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-APOBEC2 antibody [EPR11286(B)] (ab170859)

All lanes : Anti-APOBEC2 antibody [EPR11286(B)] (ab170859) at 1/10000 dilution

Lane 1 : Human fetal muscle lysate
Lane 2 : Human fetal heart lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 26 kDa
Anti-APOBEC2 antibody [EPR11286(B)] (ab170859)

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

Country/Region

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Datasheet download

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Publishing research using ab170859? Please let us know so that we can cite the reference in this datasheet.

ab170859 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Recombinant Anti-APOBEC2 antibody [EPR11286(B)] (ab170859)

Key features and details

Overview

Product name

Description

Host species

Tested Applications & Species

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Applications

The Abpromise guarantee

Target

Relevance

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (0)

Customer reviews and Q&As