Recombinant Anti-APOBEC2 antibody [EPR11286(B)] (ab170859)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11286(B)] to APOBEC2
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-APOBEC2 antibody [EPR11286(B)]
See all APOBEC2 primary antibodies -
Description
Rabbit monoclonal [EPR11286(B)] to APOBEC2 -
Host species
Rabbit -
Tested Applications & Species
Application Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human APOBEC2 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9Y235 -
Positive control
- Human heart and skeletal muscle tissues; Human fetal muscle and fetal heart lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11286(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170859 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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ICC |
Human
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IHC-P |
Human
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WB |
Human
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All applications |
Mouse
Rat
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Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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|
WB |
1/10000 - 1/50000. Predicted molecular weight: 26 kDa.
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Notes |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/10000 - 1/50000. Predicted molecular weight: 26 kDa. |
Target
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Relevance
APOBEC2 belongs to the cytidine and deoxycytidylate deaminase family. It is probable C to U editing enzyme whose physiological substrate is not yet known. It does not display detectable apoB mRNA editing and has a low intrinsic cytidine deaminase activity. -
Database links
- Entrez Gene: 10930 Human
- Entrez Gene: 11811 Mouse
- Entrez Gene: 301226 Rat
- Omim: 604797 Human
- SwissProt: Q9Y235 Human
- SwissProt: Q9WV35 Mouse
- Unigene: 555915 Human
- Unigene: 281793 Mouse
see all -
Alternative names
- APOBEC1-LIKE antibody
- Apolipoprotein B mRNA editing enzyme catalytic polypeptide 2 antibody
- Apolipoprotein B mRNA editing enzyme catalytic polypeptide like 2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (ab170859)
Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human skeletal muscle tissue labeling APOBEC2 with ab170859 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (ab170859)
Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human heart tissue labeling APOBEC2 with ab170859 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (ab170859)
ab170859 showing -ve staining in Human thyroid gland carcinoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (ab170859)
ab170859 showing -ve staining in Human normal liver tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (ab170859)
ab170859 showing -ve staining in Human normal kidney tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (ab170859)
ab170859 showing -ve staining in Human cervical carcinoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-APOBEC2 antibody [EPR11286(B)] (ab170859) at 1/10000 dilution
Lane 1 : Human fetal muscle lysate
Lane 2 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 26 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab170859 has not yet been referenced specifically in any publications.