Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab222330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

Target

  • Relevance

    APOBEC3B is a member of the cytidine deaminase gene family. However, it lacks cytidine deaminase activity, at least on RNA molecules (monomeric nucleoside substrates or synthetic apoB, NF1 and NAT1 RNA template). It binds to apoB and AU-rich RNAs. It is unable to reduce HIV-1 infectivity in vitro.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • A3B antibody
    • APOBEC1L antibody
    • Apolipoprotein B mRNA editing enzyme catalytic polypeptide like 3B antibody
    • ARCD3 antibody
    • ARP4 antibody
    • bK150C2.2 antibody
    • Cytidine deaminase antibody
    • DJ742C19.2 antibody
    • DNA dC->dU-editing enzyme APOBEC-3B antibody
    • FLJ21201 antibody
    • Phorbolin 1 related protein antibody
    • Phorbolin 2 antibody
    • Phorbolin 2/3 antibody
    • Phorbolin 3 antibody
    • PHRBNL antibody
    • Probable DNA dC>dU editing enzyme APOBEC3B antibody
    see all

Images

  • PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for APOBEC3B (green) using ab222330 at 4 µg/ml in ICC/IF.

References

ab222330 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Flow Cytometry
Sample
Human Cell (Breast)
Permeabilization
Yes - Triton 0,15% buffer
Gating Strategy
scatter characteristics of living cells
Specification
Breast
Preparation
Cell harvesting/tissue preparation method: formaldehyde-fixed cell line
Sample buffer: Triton 0,15% buffer
Fixation
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 10 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 1mM EDTA
Permeabilization
No
Specification
Colon
Blocking step
(agent) for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Oct 31 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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