Overview

  • Product name

    Anti-APOBEC3B antibody [EPR18138]
    See all APOBEC3B primary antibodies
  • Description

    Rabbit monoclonal [EPR18138] to APOBEC3B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human APOBEC3B aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9UH17

  • Positive control

    • WB:Human APOBEC3B fragment recombinant protein; HepG2, Jurkat and THP-1 whole cell lysates; Human fetal heart lysate; HepaRG-APOBEC3B tetracycline-inducible cell line; HCC1143 cell line infected with control (+) and APOBEC3B lentivirus (-); HEK293-APOBEC3B(+GFP) tetracycline-inducible cell line.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab184990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).

Target

  • Relevance

    APOBEC3B is a member of the cytidine deaminase gene family. However, it lacks cytidine deaminase activity, at least on RNA molecules (monomeric nucleoside substrates or synthetic apoB, NF1 and NAT1 RNA template). It binds to apoB and AU-rich RNAs. It is unable to reduce HIV-1 infectivity in vitro.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • A3B antibody
    • APOBEC1L antibody
    • Apolipoprotein B mRNA editing enzyme catalytic polypeptide like 3B antibody
    • ARCD3 antibody
    • ARP4 antibody
    • bK150C2.2 antibody
    • Cytidine deaminase antibody
    • DJ742C19.2 antibody
    • DNA dC->dU-editing enzyme APOBEC-3B antibody
    • FLJ21201 antibody
    • Phorbolin 1 related protein antibody
    • Phorbolin 2 antibody
    • Phorbolin 2/3 antibody
    • Phorbolin 3 antibody
    • PHRBNL antibody
    • Probable DNA dC>dU editing enzyme APOBEC3B antibody
    see all

Images

  • All lanes : Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/1000 dilution

    Lane 1 : Human APOBEC3B fragment recombinant protein
    Lane 2 : Human APOBEC3G fragment recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/1000 dilution + HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/2000 dilution + Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/1000 dilution + THP-1 (Human monocytic leukemia cell line) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/1000 dilution + Human fetal heart lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/10000 dilution

    Lane 1 : HepaRG-APOBEC3B tetracycline-inducible cell line (+/-)
    Lane 2 : HCC1143 cell line infected with control (+) and APOBEC3B lentivirus (-)
    Lane 3 : HEK293-APOBEC3B(+GFP) tetracycline-inducible cell line (+/-)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (H+L) conjugated to HRP at 1/10000 dilution

    Predicted band size: 46 kDa
    Observed band size: 35,70 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST.

    Dilution buffer: 1% NFDM/TBST.

    Exposure time: Panel A: 20 s; Panel B: 1 min.

    Figure provided by Dr. Jenni Nikkila. Gene Function Lab at The Institute of Cancer Research, London. HEK293-APOBEC3B(+GFP) tetracycline-inducible cell line provide by Dr Reuben Harris, University of Minnesota, Minneapolis - cited in Akre, M.K, G.J. Starrett, J.S. Quist, N.A. Temiz, M.A. Carpenter, A.N.J. Tutt, A. Grigoriadis & R.S. Harris (2016) Mutation processes in 293-based clones overexpressing the DNA cytosine deaminase APOBEC3B. PLoS One11(5):e0155391. doi: 10.1371/journal.pone.0155391.

    The 35 kDa band represents a known splice variant of APOBEC3B.

References

This product has been referenced in:

See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
50000 cells
Specification
Glioblastoma
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Aug 15 2019

Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma cells)
Gel Running Conditions
Reduced Denaturing (4 - 12%)
Loading amount
50000 cells
Specification
Glioblastoma cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jul 23 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (normal colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 1mM EDTA
Permeabilization
No
Specification
normal colon
Blocking step
(agent) for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Oct 09 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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