Anti-APOBEC3B antibody - N-terminal (ab191695)
Key features and details
- Rabbit polyclonal to APOBEC3B - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-APOBEC3B antibody - N-terminal
See all APOBEC3B primary antibodies -
Description
Rabbit polyclonal to APOBEC3B - N-terminal -
Host species
Rabbit -
Specificity
ab191695 will detect all three isoforms of APOBEC3B. ab191695 may cross-react with APOBEC3D. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human APOBEC3B (N terminal). The exact sequence is proprietary. Peptide corresponds to 14 amino acids near the amino terminus (NP_004891).
Database link: Q9UH17 -
Positive control
- IHC: Human small intestine tissue. WB: HepG2 cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 46 kDa).
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IHC-P |
Use a concentration of 5 µg/ml.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 46 kDa). |
IHC-P
Use a concentration of 5 µg/ml. |
Target
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Relevance
APOBEC3B is a member of the cytidine deaminase gene family. However, it lacks cytidine deaminase activity, at least on RNA molecules (monomeric nucleoside substrates or synthetic apoB, NF1 and NAT1 RNA template). It binds to apoB and AU-rich RNAs. It is unable to reduce HIV-1 infectivity in vitro. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 9582 Human
- Omim: 607110 Human
- SwissProt: Q9UH17 Human
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Alternative names
- A3B antibody
- APOBEC1L antibody
- Apolipoprotein B mRNA editing enzyme catalytic polypeptide like 3B antibody
see all
Images
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Anti-APOBEC3B antibody - N-terminal (ab191695) at 1 µg/ml + HepG2 cell lysate
Predicted band size: 46 kDa -
Immunohistochemical analysis of human small intestine tissue, labeling APOBEC3B using ab191695 at 20 μg/mL.
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Immunohistochemical analysis of paraffin-embedded Human small intestine tissue, labeling APOBEC3B using ab191695 at 5 μg/mL.
Protocols
Datasheets and documents
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Datasheet download
References (7)
ab191695 has been referenced in 7 publications.
- Zhang X et al. Aberrantly Activated APOBEC3B Is Associated With Mutant p53-Driven Refractory/Relapsed Diffuse Large B-Cell Lymphoma. Front Immunol 13:888250 (2022). PubMed: 35592333
- He L et al. Prognostic Value of HPV E6 and APOBEC3B in Upper Urinary Tract Urothelial Carcinoma. Dis Markers 2022:2147494 (2022). PubMed: 35903294
- Gara SK et al. GATA3 and APOBEC3B are prognostic markers in adrenocortical carcinoma and APOBEC3B is directly transcriptionally regulated by GATA3. Oncotarget 11:3354-3370 (2020). PubMed: 32934779
- Mao Y et al. APOBEC3B expression and its prognostic potential in breast cancer. Oncol Lett 19:3205-3214 (2020). PubMed: 32256817
- Feng C et al. APOBEC3B High Expression in Gastroenteropancreatic Neuroendocrine Neoplasms and Association With Lymph Metastasis. Appl Immunohistochem Mol Morphol 27:599-605 (2019). PubMed: 30095460
- Hwang JA et al. Genomic profiles of lung cancer associated with idiopathic pulmonary fibrosis. J Pathol 244:25-35 (2018). PubMed: 28862766
- Sieuwerts AM et al. Progressive APOBEC3B mRNA expression in distant breast cancer metastases. PLoS One 12:e0171343 (2017). PubMed: 28141868