Recombinant Anti-APOBEC3C/PBI antibody [EPR13417] (ab181356)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13417] to APOBEC3C/PBI
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-APOBEC3C/PBI antibody [EPR13417]
See all APOBEC3C/PBI primary antibodies -
Description
Rabbit monoclonal [EPR13417] to APOBEC3C/PBI -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Jurkat, Daudi, K562, and HeLa whole cell lysate (ab150035); HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13417 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/5000. Predicted molecular weight: 23 kDa.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/5000. Predicted molecular weight: 23 kDa. |
Target
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Function
Host cellular restriction factor that may have antiviral activities against exogenous and endogenous viruses, as well as retrotransposons. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation. -
Tissue specificity
Expressed in spleen, testes, peripherical blood lymphocytes, heart, thymus, prostate and ovary. -
Sequence similarities
Belongs to the cytidine and deoxycytidylate deaminase family. - Information by UniProt
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Database links
- Entrez Gene: 27350 Human
- Omim: 607750 Human
- SwissProt: Q9NRW3 Human
- Unigene: 441124 Human
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Alternative names
- A3C antibody
- ABC3C_HUMAN antibody
- APOBEC1 like antibody
see all
Images
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Intracellular flow cytometric analysis of permeabilized HeLa cells labeling APOBEC3C/PBI with ab181356 at 1/10 dilution (red) compared toa rabbit IgG negative control (green).
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All lanes : Anti-APOBEC3C/PBI antibody [EPR13417] (ab181356) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Daudi cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 23 kDa
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab181356 has been referenced in 2 publications.
- Tao L et al. Induction of APOBEC3C Facilitates the Genotoxic Stress-Mediated Cytotoxicity of Artesunate. Chem Res Toxicol 32:2526-2537 (2019). PubMed: 31664825
- Anderson BD et al. Natural APOBEC3C variants can elicit differential HIV-1 restriction activity. Retrovirology 15:78 (2018). PubMed: 30558640