Overview

  • Product name

  • Description

    Goat polyclonal to APOBEC3D
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    C-PKRQSNHRQEVYFR

    , corresponding to internal sequence amino acids 61-74 of Human APOBEC3D (NP_689639.2).

  • Positive control

    • A549 cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab115021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 47 kDa).

Target

  • Function

    Unknown. Unable to reduce HIV-1 infectivity in vitro.
  • Sequence similarities

    Belongs to the cytidine and deoxycytidylate deaminase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • A3D antibody
    • ABC3D_HUMAN antibody
    • APOBEC3D antibody
    • APOBEC3DE antibody
    • APOBEC3E antibody
    • Apolipoprotein B mRNA editing enzyme catalytic polypeptide like 3D antibody
    • Apolipoprotein B mRNA editing enzyme catalytic polypeptide like 3E pseudogene antibody
    • ARP6 antibody
    • bK150C2.9 antibody
    • BK150C2.9 protein antibody
    • DNA dC >dU editing enzyme APOBEC 3D antibody
    • DNA dC->dU-editing enzyme APOBEC-3D antibody
    • hCG_41704 antibody
    • HCG41704, isoform CRA_b antibody
    • Probable DNA dC->dU-editing enzyme APOBEC-3D antibody
    see all

Images

  • Anti-APOBEC3D antibody (ab115021) at 0.3 µg/ml + A549 lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 47 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 hour

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for your response and for attaching the images.
This is to let you know that as you requested I have placed a new order for you - for one vial of ab105869 as a free of charge replacement (alternative for ab115021) and the new order number is 1081741. Currently, this product is out of stock and it may take a few days to get it from our Lab.
I hope the second vial will work as it is expected, and please do let me know how you are getting on with this product.

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Answer

Thank you very much for your updates. Your time and effort would be very much appreciated
I wonder if you could send a WB image so that I can take a look at the different band size(s)? It may well that endogenously-expressed and transfected protein runs at different size on the gel.
I could certainly offer you a new vial from a different antibody ab105869 (as a free of charge replacement - FOCR). This alternative antibody is from a different source; the host species, the immunogen used and the testing conditions were different. Please could you take a look at the datasheet and confirm to me if you wish to get a FOCR.
I look forward to hearing from you soon.

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Answer

Thank you for your response and your comments.
Northern blot analysis detected APOBEC3D expression in colorectal adenocarcinoma cells, chronic myelogenous leukemia cells, lung carcinoma and HeLa cells. No expression was detected in normal tissues. The cell line A549 is a lung carcinoma cell line.
You may wish to take a look a this site for further information about the expression profile of APOBEC3D:
http://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer.cgi?uglist=Hs.658626
Q1: Does the construct contain full sequence or fragment of the target protein?
Q2: Have you used any tagging which may interact with the recognition of the epitope?
Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.

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Answer

Thank you for your enquiry regarding ab115021 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.
I would like to reassure you that our Abpromise applies to your complaint since you purchased this product within the guarantee period. This means that in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.
Though you have kindly provided some details, it would be much appreciated if I could get some more information which would help me identify the source of the problem.
1) Did the blot without primary come out clean?
2) Have you run a positive control suggested on the datasheet i.e. A549 lysate?
Thank you for your understanding and co-operation in this matter. I look forward to hearing from you and hope to solve this problem as soon as possible.

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