Overview

  • Product name

  • Description

    Rabbit polyclonal to APOBEC3F
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human APOBEC3F (internal sequence). The exact sequence is proprietary.
    Database link: Q8IUX4

  • Positive control

    • WB: Jurkat whole cell lysate (ab7899). IHC-P: Human colon carcinoma tissue. ICC/IF: A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 46 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.

Target

  • Function

    DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. Exhibits antiviral activity against vif-deficient HIV-1. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA. Exhibits antiviral activity also against hepatitis B virus (HBV), equine infectious anemia virus (EIAV), xenotropic MuLV-related virus (XMRV) and simian foamy virus (SFV) and may inhibit the mobility of LTR and non-LTR retrotransposons. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation.
  • Tissue specificity

    Widely expressed. Highly expressed in ovary.
  • Sequence similarities

    Belongs to the cytidine and deoxycytidylate deaminase family.
    Contains 2 CMP/dCMP-type deaminase domains.
  • Domain

    The CMP/dCMP deaminase domain 1 mediates RNA binding, RNA-dependent oligomerization and virion incorporation whereas the CMP/dCMP deaminase domain 2 confers deoxycytidine deaminase activity and substrate sequence specificity.
  • Cellular localization

    Cytoplasm. Cytoplasm, P-body.
  • Information by UniProt
  • Database links

  • Alternative names

    • A3F antibody
    • ABC3F_HUMAN antibody
    • APOBEC3F antibody
    • Apolipoprotein B mRNA editing enzyme, catalytic polypeptide like 3F antibody
    • Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F antibody
    • ARP8 antibody
    • DNA dC->dU-editing enzyme APOBEC-3F antibody
    • Induced upon T cell activation antibody
    • KA6 antibody
    see all

Images

  • Anti-APOBEC3F antibody (ab227962) at 1/1000 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg

    Predicted band size: 46 kDa



    10% SDS PAGE gel.

  • Paraffin-embedded human colon carcinoma tissue stained for APOBEC3F using ab227962 at 1/250 dilution in immunohistochemical analysis.

  • Paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for APOBEC3F (green) using ab227962 at 1/500 dilution in ICC/IF.

    Nuclear counter stain: Hoechst 33342 (blue).

References

ab227962 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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