Key features and details
- Goat polyclonal to APOBEC3G/A3G
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-APOBEC3G/A3G antibody
See all APOBEC3G/A3G primary antibodies
DescriptionGoat polyclonal to APOBEC3G/A3G
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Cynomolgus monkey
- Daudi cell lysate, Human kidney and Human testis tissues
Previously labelled as APOBEC3G
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab109727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 46 kDa).|
|IHC-P||Use a concentration of 2.5 µg/ml.|
FunctionDNA deaminase (cytidine deaminase) that mediates a form of innate resistance to retroviral infections (at least to HIV-1 infection) by triggering G-to-A hypermutation in the newly synthesized viral DNA. The replacements C-to-U in the minus strand DNA of HIV-1 during reverse transcription, leads to G-to-A transitions in the plus strand. The inhibition of viral replication is either due to the degradation of the minus strand before its integration or to the lethality of the hypermutations. Modification of both DNA strands is not excluded. This antiviral activity is neutralized by the virion infectivity factor (VIF), that prevents the incorporation of APOBEC3G into progeny HIV-1 virions by both inhibiting its translation and/or by inducing its ubiquitination and subsequent degradation by the 26S proteasome. APOBEC3G binds a variety of RNAs, but does not display detectable APOB, NF1 and NAT1 mRNA editing.
Tissue specificityExpressed in spleen, testes, ovary and peripheral blood leukocytes and CD4+ lymphocytes. Also expressed in non-permissive peripheral blood mononuclear cells, and several tumor cell lines; no expression detected in permissive lymphoid and non-lymphoid cell lines.
Sequence similaritiesBelongs to the cytidine and deoxycytidylate deaminase family.
modificationsUbiquitinated in the presence of HIV-1 VIF. Association with VIF targets the protein for proteolysis by the ubiquitin-dependent proteasome pathway.
Cellular localizationCytoplasm. Nucleus. Mainly cytoplasmic. Small amount are found in the nucleus. During HIV-1 infection, virion-encapsidated in absence of HIV-1 VIF.
- Information by UniProt
- A3G antibody
- ABC3G_HUMAN antibody
- APOBEC related cytidine deaminase antibody
Anti-APOBEC3G/A3G antibody (ab109727) at 0.5 µg/ml + Daudi cell lysate (in RIPA buffer) at 35 µg/ml
Developed using the ECL technique.
Predicted band size: 46 kDa
Primary incubation was 1 hour
ab109727 at a concentration of 2.5 µg/ml staining APOBEC3G/A3G in Human Kidney, Formalin-Fixed, Paraffin-Embedded section.
ab109727 at a concentration of 2.5 µg/ml staining APOBEC3G/A3G in Human Testis, Formalin-Fixed, Paraffin-Embedded section.
ab109727 has not yet been referenced specifically in any publications.