- References (4)
Product nameApocynin, NADPH-oxidase inhibitor
DescriptionSelective NADPH-oxidase inhibitor
Biological descriptionSelective NADPH-oxidase inhibitor (IC50 = 10 μM). Inhibits production of reactive oxygen species. Also elicits a range of in vitro and in vivo anti-inflammatory effects.
Storage instructionsStore at Room Temperature. The product can be stored for up to 12 months.
Solubility overviewSoluble in DMSO to 100 mM and in ethanol to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Our Abpromise guarantee covers the use of ab120615 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab19534 staining glutathione in A549 cells treated with apocynin (ab120615), by ICC/IF. Increase in glutathione expression correlates with increased concentration of apocynin, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120615 (apocynin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab19534 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody.
A–B. Endothelial cells were transfected with Nef cDNA, incubated for further 6 hours and treated with apocynin (200 nM), trolox (200 nM), Nox2 inhibitor (1 uM) or IKKi (100 nM). After additional 18 hours supernatants were analyzed for Nef-induced MCP-1 production (A) and endothelial cells for apoptosis using TUNEL (B). C–D. Endothelial cells were cocultured with Nef-transfected Jurkat cells for 24 h, and then treated with apocynin (200 nM), trolox (200 nM), Nox2 inhibitor (1 uM) or IKKi (100 nM) and incubated an additional 18 h, then analyzed for Nef-induced MCP-1 production (C) and apoptosis of endothelial cells (D). Data were expressed as fold MCP-1 production and apoptosis, normalized to the mean of control measurements. Data represent mean±SD from 3 separate experiments in which measurements were made in triplicate. *P<0.05, and **P<0.01.
Wang T et al., PloS one., 9(3): e91063. Fig 6.; doi: 10.1371/journal.pone.0091063
This product has been referenced in:
- Genovese T et al. Modulation of NADPH oxidase activation in cerebral ischemia/reperfusion injury in rats. Brain Res 1372:92-102 (2011). Read more (PubMed: 21138737) »
- Impellizzeri D et al. Effect of apocynin, a NADPH oxidase inhibitor, on acute lung inflammation. Biochem Pharmacol 81:636-48 (2011). Read more (PubMed: 21147071) »
- Impellizzeri D et al. Effect of Apocynin, an inhibitor of NADPH oxidase, in the inflammatory process induced by an experimental model of spinal cord injury. Free Radic Res 45:221-36 (2011). Read more (PubMed: 20954833) »