Key features and details
- Rabbit polyclonal to ApoER2
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ApoER2 antibody
See all ApoER2 primary antibodies
DescriptionRabbit polyclonal to ApoER2
SpecificitySpecific for ApoER2 idoforms 1,2 and 4
Tested applicationsSuitable for: WBmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide derived from the C-terminal region of the human ApoER2 protein
- Human placenta homogenates Mouse brain homogenates
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab52905 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 154 kDa (predicted molecular weight: 106 kDa).|
FunctionCell surface receptor for Reelin (RELN) and apolipoprotein E (apoE)-containing ligands. LRP8 participates in transmitting the extracellular Reelin signal to intracellular signaling processes, by binding to DAB1 on its cytoplasmic tail. Reelin acts via both the VLDL receptor (VLDLR) and LRP8 to regulate DAB1 tyrosine phosphorylation and microtubule function in neurons. LRP8 has higher affinity for Reelin than VLDLR. LRP8 is thus a key component of the Reelin pathway which governs neuronal layering of the forebrain during embryonic brain development. Binds the endoplasmic reticulum resident receptor-associated protein (RAP). Binds dimers of beta 2-glycoprotein I and may be involved in the suppression of platelet aggregation in the vasculature. Highly expressed in the initial segment of the epididymis, where it affects the functional expression of clusterin and phospholipid hydroperoxide glutathione peroxidase (PHGPx), two proteins required for sperm maturation. May also function as an endocytic receptor.
Tissue specificityExpressed mainly in brain and placenta. Also expressed in platelets and megakaryocytic cells. Not expressed in the liver.
Involvement in diseaseDefects in LRP8 are a cause of myocardial infarction type 1 (MCI1) [MIM:608446]. A condition defined by the irreversible necrosis of heart muscle secondary to prolonged ischemia.
Sequence similaritiesBelongs to the LDLR family.
Contains 2 EGF-like domains.
Contains 7 LDL-receptor class A domains.
Contains 5 LDL-receptor class B repeats.
DomainThe cytoplasmic domain is involved in the binding of DAB1 and in the recruitment of JNK-interacting proteins. Isoforms, which lack part of the cytoplasmic domain, are unable to recruit members of the family of JNK interacting proteins (JIP) to the cytoplasmic tail.
modificationsO-glycosylated. Some alternatively spliced isoforms lack the O-linked sugar domain.
Undergoes sequential, furin and gamma-secretase dependent, proteolytic processing, resulting in the extracellular release of the entire ligand-binding domain as a soluble polypeptide and in the intracellular domain (ICD) release into the cytoplasm. The gamma-secretase-dependent proteolytical processing occurs after the bulk of the extracellular domain has been shed, in a furin-dependent manner, in alternatively spliced isoforms carrying the furin cleavage site. Hypoglycosylation (mainly hypo-O-glycosylation) leads to increased extracellular cleavage, which in turn results in accelerating release of the intracellular domain (ICD) by the gamma-secretase. The resulting receptor fragment is able to inhibit Reelin signaling and in particular the Reelin-induced DAB1 phosphorylation.
Tyrosine phosphorylated upon apoE binding.
Ubiquitinated by MYLIP leading to degradation.
Cellular localizationCell membrane. Secreted. Isoforms that contain the exon coding for a furin-type cleavage site are proteolytically processed, leading to a secreted receptor fragment.
- Information by UniProt
- APOER2 antibody
- Apolipoprotein E receptor 2 antibody
- low density lipoprotein receptor-related protein 8 antibody
All lanes : Anti-ApoER2 antibody (ab52905) at 3 µg/ml
Lane 1 : Human placenta
Lane 2 : Mouse brain homogenate
Predicted band size: 106 kDa
Observed band size: 154 kDa why is the actual band size different from the predicted?
ab52905 has been referenced in 5 publications.
- DeVay RM et al. Common Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) Epitopes Mediate Multiple Routes for Internalization and Function. PLoS One 10:e0125127 (2015). WB . PubMed: 25905719
- Masyuk AI et al. Ciliary subcellular localization of TGR5 determines the cholangiocyte functional response to bile acid signaling. Am J Physiol Gastrointest Liver Physiol 304:G1013-24 (2013). PubMed: 23578785
- Chen X et al. Up-regulation of ATP Binding Cassette Transporter A1 Expression by Very Low Density Lipoprotein Receptor and Apolipoprotein E Receptor 2. J Biol Chem 287:3751-9 (2012). WB ; Mouse . PubMed: 22170052
- Herring A et al. Exercise during pregnancy mitigates Alzheimer-like pathology in mouse offspring. FASEB J : (2011). WB ; Mouse . PubMed: 21948247
- ElAli A & Hermann DM Apolipoprotein E controls ATP-binding cassette transporters in the ischemic brain. Sci Signal 3:ra72 (2010). WB . PubMed: 20923933