Recombinant Anti-ApoER2 antibody [EPR3326] - BSA and Azide free (ab215274)


  • Product name

    Anti-ApoER2 antibody [EPR3326] - BSA and Azide free
    See all ApoER2 primary antibodies
  • Description

    Rabbit monoclonal [EPR3326] to ApoER2 - BSA and Azide free
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human ApoER2 aa 950 to the C-terminus (C terminal).
    Database link: Q14114
    (Peptide available as ab193569)

  • Positive control

    • WB: SH-SY5Y, C6 and Neuro-2a whole cell lysates; Human fetal brain lysate; Mouse brain tissue lysate.
  • General notes

    Ab215274 is the carrier-free version of ab108208. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab215274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 106 kDa.
IP Use at an assay dependent concentration.


  • Function

    Cell surface receptor for Reelin (RELN) and apolipoprotein E (apoE)-containing ligands. LRP8 participates in transmitting the extracellular Reelin signal to intracellular signaling processes, by binding to DAB1 on its cytoplasmic tail. Reelin acts via both the VLDL receptor (VLDLR) and LRP8 to regulate DAB1 tyrosine phosphorylation and microtubule function in neurons. LRP8 has higher affinity for Reelin than VLDLR. LRP8 is thus a key component of the Reelin pathway which governs neuronal layering of the forebrain during embryonic brain development. Binds the endoplasmic reticulum resident receptor-associated protein (RAP). Binds dimers of beta 2-glycoprotein I and may be involved in the suppression of platelet aggregation in the vasculature. Highly expressed in the initial segment of the epididymis, where it affects the functional expression of clusterin and phospholipid hydroperoxide glutathione peroxidase (PHGPx), two proteins required for sperm maturation. May also function as an endocytic receptor.
  • Tissue specificity

    Expressed mainly in brain and placenta. Also expressed in platelets and megakaryocytic cells. Not expressed in the liver.
  • Involvement in disease

    Defects in LRP8 are a cause of myocardial infarction type 1 (MCI1) [MIM:608446]. A condition defined by the irreversible necrosis of heart muscle secondary to prolonged ischemia.
  • Sequence similarities

    Belongs to the LDLR family.
    Contains 2 EGF-like domains.
    Contains 7 LDL-receptor class A domains.
    Contains 5 LDL-receptor class B repeats.
  • Domain

    The cytoplasmic domain is involved in the binding of DAB1 and in the recruitment of JNK-interacting proteins. Isoforms, which lack part of the cytoplasmic domain, are unable to recruit members of the family of JNK interacting proteins (JIP) to the cytoplasmic tail.
  • Post-translational

    O-glycosylated. Some alternatively spliced isoforms lack the O-linked sugar domain.
    Undergoes sequential, furin and gamma-secretase dependent, proteolytic processing, resulting in the extracellular release of the entire ligand-binding domain as a soluble polypeptide and in the intracellular domain (ICD) release into the cytoplasm. The gamma-secretase-dependent proteolytical processing occurs after the bulk of the extracellular domain has been shed, in a furin-dependent manner, in alternatively spliced isoforms carrying the furin cleavage site. Hypoglycosylation (mainly hypo-O-glycosylation) leads to increased extracellular cleavage, which in turn results in accelerating release of the intracellular domain (ICD) by the gamma-secretase. The resulting receptor fragment is able to inhibit Reelin signaling and in particular the Reelin-induced DAB1 phosphorylation.
    Tyrosine phosphorylated upon apoE binding.
    Ubiquitinated by MYLIP leading to degradation.
  • Cellular localization

    Cell membrane. Secreted. Isoforms that contain the exon coding for a furin-type cleavage site are proteolytically processed, leading to a secreted receptor fragment.
  • Information by UniProt
  • Database links

  • Alternative names

    • APOER2 antibody
    • Apolipoprotein E receptor 2 antibody
    • low density lipoprotein receptor-related protein 8 antibody
    • Low-density lipoprotein receptor-related protein 8 antibody
    • LRP-8 antibody
    • LRP8 antibody
    • LRP8_HUMAN antibody
    see all


  • ab108208 (purified) at 1/40 immunoprecipitating ApoER2 in SH-SY5Y (human neuroblastoma) whole cell lysate.

    Lane 1 (input): SH-SY5Y whole cell lysate 10ug

    Lane 2 (+): ab108208 + SH-SY5Y whole cell lysate

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108208 in SH-SY5Y whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108208).

  • All lanes : Anti-ApoER2 antibody [EPR3326] (ab108208) at 1/10000 dilution

    Lane 1 : SH-SY5Y (human neuroblastoma) whole cell lysate
    Lane 2 : Human fetal brain tissue lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : C6 (rat glioma) whole cell lysate
    Lane 5 : Neuro-2a (mouse neuroblastoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG

    Predicted band size: 106 kDa

    Blocking and diluting buffer 5% NFDM/TBST.

    130kDa: Immature form;

    170kDa: Glycosylated mature form. (PMID:24344333, 24532792, 24705369 and 25233900)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108208).


ab215274 has not yet been referenced specifically in any publications.

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