Recombinant Anti-APOF antibody [EPR2908] (ab92301)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-APOF antibody [EPR2908]
See all APOF primary antibodies -
Description
Rabbit monoclonal [EPR2908] to APOF -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICCmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human APOF aa 150-250. The exact sequence is proprietary.
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Positive control
- HDL and Human plasma lysate
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product was previously labelled as Apolipoprotein F
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2908 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab92301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 33 kDa. | |
| ICC | 1/100 - 1/250. |
Target
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Function
Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 319 Human
- Omim: 107760 Human
- SwissProt: Q13790 Human
- Unigene: 534302 Human
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Alternative names
- Apo-F antibody
- Apof antibody
- APOF_HUMAN antibody
see all
Images
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![Western blot - Anti-APOF antibody [EPR2908] (ab92301)](https://www.abcam.com/ps/products/92/ab92301/Images/Apolipoprotein-F-Primary-antibodies-ab92301-2.jpg)
Western blot - Anti-APOF antibody [EPR2908] (ab92301)All lanes : Anti-APOF antibody [EPR2908] (ab92301) at 1/2000 dilution
Lane 1 : HDL lysate
Lane 2 : Human plasma lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 33 kDa
Datasheets and documents
References
This product has been referenced in:
- Xu S et al. Metformin Suppresses Tumor Progression by Inactivating Stromal Fibroblasts in Ovarian Cancer. Mol Cancer Ther 17:1291-1302 (2018). Read more (PubMed: 29545331) »
