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Cardiovascular Lipids / Lipoproteins Lipid Metabolism Cholesterol Metabolism
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RecombinantRabMAb

Recombinant Anti-APOF antibody [EPR2908] (ab92301)

  • Datasheet
  • SDS
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Western blot - Anti-APOF antibody [EPR2908] (ab92301)
  • Anti-APOF antibody [EPR2908] (ab92301)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2908] to APOF
  • Suitable for: WB
  • Reacts with: Human

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Protein
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Recombinant Human APOF protein (ab116968)
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Overview

  • Product name

    Anti-APOF antibody [EPR2908]
    See all APOF primary antibodies
  • Description

    Rabbit monoclonal [EPR2908] to APOF
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human APOF aa 150-250. The exact sequence is proprietary.

  • Positive control

    • HDL and Human plasma lysate
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

     This product was previously labelled as Apolipoprotein F

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR2908
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cardiovascular
    • Atherosclerosis
    • Lipid transport
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Alternative Versions

    • Anti-APOF antibody [EPR2908] - BSA and Azide free (ab232035)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human APOF protein (ab116968)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab92301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII.
    • Tissue specificity

      Expressed by the liver and secreted in plasma.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession Q13790 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 319 Human
      • Omim: 107760 Human
      • SwissProt: Q13790 Human
      • Unigene: 534302 Human
      • Alternative names

        • Apo-F antibody
        • Apof antibody
        • APOF_HUMAN antibody
        • Apolipoprotein F antibody
        • DKFZp781G18150 antibody
        • Lipid transfer inhibitor protein antibody
        • LTIP antibody
        • MGC22520 antibody
        see all

      Images

      • Western blot - Anti-APOF antibody [EPR2908] (ab92301)
        Western blot - Anti-APOF antibody [EPR2908] (ab92301)
        All lanes : Anti-APOF antibody [EPR2908] (ab92301) at 1/2000 dilution

        Lane 1 : HDL lysate
        Lane 2 : Human plasma lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 33 kDa

      • Anti-APOF antibody [EPR2908] (ab92301)
        Anti-APOF antibody [EPR2908] (ab92301)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab92301? Please let us know so that we can cite the reference in this datasheet.

      ab92301 has been referenced in 1 publication.

      • Xu S  et al. Metformin Suppresses Tumor Progression by Inactivating Stromal Fibroblasts in Ovarian Cancer. Mol Cancer Ther 17:1291-1302 (2018). PubMed: 29545331

      Customer reviews and Q&As

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      For licensing inquiries, please contact partnerships@abcam.com

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